Cd127 clone hil 7r m21

Manufactured by BD

Sourced in United States

The CD127 (clone hIL-7R-M21) is a laboratory reagent used for the detection and analysis of CD127, also known as the interleukin-7 receptor alpha (IL-7Rα). It is a cell surface marker that is expressed on various immune cells. The CD127 antibody can be used in flow cytometry applications to identify and quantify cells expressing this receptor.

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Lab products found in correlation

Cd3 clone sp34 2, by BD (1 mentions) Hla dr clone l243, by BD (1 mentions) Pd 1 clone eh12.2h7, by BioLegend (1 mentions) Foxp3 clone pch101, by Thermo Fisher Scientific (1 mentions) Ki67 clone b56, by BD (1 mentions) Cd28 clone cd28, by Beckman Coulter (1 mentions) Lsr 2 flow cytometer, by BD (1 mentions) Cd4 clone l200, by BD (1 mentions) Cd8 clone 3b5, by Thermo Fisher Scientific (1 mentions) Cd95 clone dx2, by BioLegend (1 mentions) Cd45ro clone uchl1, by Thermo Fisher Scientific (1 mentions) Ccr6 clone 11a9, by BD (1 mentions) Cd4 clone rpa t4, by BD (1 mentions) Cd45ro clone uchl1, by BD (1 mentions) Cd4 clone sk3, by BD (1 mentions) Lsrfortessa flow cytometer, by BD (1 mentions) Fixable viability dye efluor 506, by Thermo Fisher Scientific (1 mentions) Cd3 clone ucht1, by BD (1 mentions) Foxp3 clone 236a e7, by BD (1 mentions) Lsrii flow cytometer, by BD (1 mentions)

Spelling variants of this product

HIL-7R-M21 (16 citations) Clone HIL-7R-M21 (5 citations) CD127 (HIL-7R-M21) (4 citations) CD127-PE (clone hIL-7R-M21), (4 citations) Anti-CD127 AlexaFluor647 (clone HIL-7R-M21) (3 citations) Anti-CD127 (clone HIL-7R-M21, (2 citations) CD127-biotin clone HIL-7R-M21 (2 citations)

3 protocols using cd127 clone hil 7r m21

Multiparameter Phenotypic Characterization of PBMCs

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Thawed PBMCs were surface stained with Aqua amine reactive viability dye (Invitrogen) and mAbs to CD45 (clone D058–1283, BD Biosciences), CD3 (clone SP34–2, BD Biosciences), CD4 (clone L200, BD Biosciences), CD8 (clone 3B5, Invitrogen), CD95 (clone DX2, BioLegend), CD28 (clone CD28.2, Beckman), CD127 (clone hIL-7R-M21, BD Biosciences), PD-1 (clone EH12.2H7, BioLegend), and HLA-DR (clone L243, BD Biosciences) for 20 min at room temperature, fixed, and then permeabilized with FoxP3 Fix/Perm Buffers (eBioscience) per the manufacturer’s instructions. Permeabilized cells were then stained intracellularly for Ki67 (clone B56, BD Biosciences) and FoxP3 (clone PCH101, eBioscience) for 30 min at 4°C, washed in FoxP3 Perm/Wash buffer, fixed in 0.5% paraformaldehyde and analyzed by flow cytometry on a BD LSRII flow cytometer (BD Biosciences) and analyzed using Flowjo Software (Tree Star Inc).

Swainson L.A., Ahn H., Pajanirassa P., Khetarpal V., Deleage C., Estes J.D., Hunt P.W., Munoz-Sanjuan I, & McCune J.M. (2019). Kynurenine 3-monooxygenase inhibition during acute SIV infection lowers PD-1 expression and improves post-cART CD4+ T cell counts and body weight: KMO inhibition improves clinical outcome in SIV infection. Journal of immunology (Baltimore, Md. : 1950), 203(4), 899-910.

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Phenotyping of Skin-Homing T Cells

Cited 1 time

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PBMCs were isolated from peripheral blood through ficoll and were stored in liquid nitrogen until use. Monoclonal antibody staining of PBMCs was performed as described earlier [9 (link)]. Briefly, cells were incubated for 15 min at room temperature with either the first panel including human antibodies: CCR10 (clone 314305; R&D), CD45RO (clone UCHL1; BD Biosciences), CCR6 (clone 11A9; BD Biosciences), CCR4 (clone 1G1; BD Biosciences), CD3 (clone UCHT1; BD Biosciences), cutaneous leukocyte antigen (CLA, clone HECA-452; BD Biosciences), CD4 (clone RPA-T4; BD Biosciences), and CXCR3 (clone G025H7; Sony), CD25 (clone bc96; Sony). CLA was added to the panel to visualize skin-homing T cells. Or cells were incubated with the second panel including CD3 (clone UCHT1; BD Biosciences), CD4 (clone SK3; BD Biosciences), CD25 (clone bc96; Sony), CD45RO (clone UCHL1; eBioscience), CD127 (clone hIL-7R-M21;BD Biosciences), and CLA (clone HECA-452; BD Biosciences). Subsequently, dead cells were excluded by incubation with Fixable Viability Dye eFluor506 (eBioscience) for 15 min at 4°C. All incubation steps were performed in the dark. Samples were measured on an LSRFortessa flow cytometer (BD Biosciences). Data were analyzed manually using FlowJo v10.7 software (Tree Star Inc. Ashland, OR). Additional file 1A and B depict the manual gating strategy of the two panels.

den Braanker H., Razawy W., Wervers K., Mus A.M., Davelaar N., Kok M.R, & Lubberts E. (2022). Characterizing memory T helper cells in patients with psoriasis, subclinical, or early psoriatic arthritis using a machine learning algorithm. Arthritis Research & Therapy, 24, 28.

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T-Cell Isolation and Phenotyping

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Prior to and following magnetic bead isolation procedures, T cells were analyzed using flow cytometry to assess the efficiency of T-cell isolation procedures. To permit T cell phenotyping, cells were washed and then stained for surface expression of CD4 (BD Pharmingen, San Diego, USA), CD25 (BD Biosciences, San Jose, USA) and CD127 (clone hIL-7R-M21) (BD Pharmingen, San Diego, USA) followed by intra-cellular staining for FOXP3 (Clone 236A/E7) (BD Pharmingen, San Diego, USA). Flow cytometry was performed using an LSR II flow cytometer (BD Biosciences, San Jose, USA). Cytobank software was used to analyze data generated from flow cytometry. Following bead enrichment, cells were harvested and washed twice with ice-cold PBS, snap-frozen in liquid nitrogen and then stored at −80 until further sample preparation.

Olsson N., Schultz L.M., Zhang L., Khodadoust M.S., Narayan R., Czerwinski D.K., Levy R, & Elias J.E. (2018). T-cell immunopeptidomes reveal cell subtype surface markers derived from intracellular proteins. Proteomics, 18(12), e1700410.

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