Taxifolin, daidzein, genistein, and equol were bought from Extrasynthese (France), and acetic acid was obtained from Fluka (UK). Methanol HPLC grade was bought from Fisher Scientific (UK), and sodium dihydrogen orthophosphate was obtained from BDH (Analar). All other chemicals and enzymes for hydrolysis reaction were bought from Sigma (UK).
Sodium dihydrogen orthophosphate
Manufactured by BD
Sourced in United Kingdom
Sodium dihydrogen orthophosphate is a chemical compound with the formula NaH2PO4. It is a crystalline solid that is soluble in water and is commonly used as a pH buffer in laboratory settings.
Automatically generated - may contain errors
Lab products found in correlation
Hplc grade methanol, by Thermo Fisher Scientific (1 mentions)
Daidzein, by Extrasynthese (1 mentions)
Genistein, by Extrasynthese (1 mentions)
Equol, by Extrasynthese (1 mentions)
Taxifolin, by Extrasynthese (1 mentions)
Bovine lactoferrin, by Merck Group (1 mentions)
Lactose, by Merck Group (1 mentions)
2 protocols using sodium dihydrogen orthophosphate
1
Bioactive Compound Extraction Protocol
2
Lactoferrin and β-Lactoglobulin Modification
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Disodium hydrogen orthophosphate and sodium dihydrogen orthophosphate were from BDH (Poole, UK). Bovine lactoferrin, β-LG, and lactose and all other general chemicals were obtained from Sigma (St. Louis, MO). Lactoferrin and β-LG were each dissolved in PBS (10 mM, pH 6.8) at a concentration of 0.15 mM (11.1 mg/mL of lactoferrin, 2.67 mg/mL of LG). The solutions were heated at 55°C in the presence or absence of lactose (146 mM) with agitation using a Mini LabRoller rotator (Labnet, Woodbridge, NJ) for 24, 48, 72, 96, 120, 144, and 168 h. This resulted in a 973-fold molar ratio excess of lactose:protein, reflecting a biologically relevant lactose concentration and the lactose:substrate ratios used in Dalsgaard et al. (2007) and Meltretter et al. (2007) .
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