Whatman filters size 1

In this study, the plant Annona muricata used in this study is not a globally threatened species. It was prepared by the department of biotechnology/ university of Technology from the local market in the city of Baghdad/Iraq. The phytochemical extraction was performed using 2 L chilled disinfected deionized distilled water mixed with 500 g A. muricata peel. To facilitate phytochemical removal uniformity, the sample was subjected to periodic vortexing during the 120-h standing period. Whatman filters (size 1) were used for staining the aqueous solution using a vacuum pump. A 4000-efficient Heldolph Laborata rotary evaporation apparatus was used for vacuum-based concentration for 12 h at 40 °C. Evaporation apparatus was used to dry the concentrates, and a powder form was obtained by treating the samples in hot air ovens set at 40 °C. The dry powder specimens obtained from the peel extracts were preserved at 4 °C using dark containers until used.

For each powder type, 500 g were mixed thoroughly with 2 L of chilled sterile distilled deionized water in dark extraction bottles and left to stand for 120 h but with occasional vortexing to ensure uniform phytochemical extraction. The aqueous mixtures were strained through Whatman filters size 1 with the aid of a vacuum suck pump and concentrated under vacuum using a Heldolph Laborata 4000-efficient rotary evaporator at 40°C for 12 h. The concentrates were transferred to evaporation pans and dried to a powder by a hot air oven set at 40°C. The obtained dry powders from each plant extract (PE) were stored in dark containers at 4°C until further use.