To visualize Cep78, Ift20, and Ttc21a, NIH3T3 cells were stained with Cep78 (custom-made against antigen p457-741 of mouse Cep78 NP_932136.2), Ift20 (Proteintech), and Ttc21a (Abnova) antibodies (Table 5) for immunofluorescence. For SIM imaging, then photographed by an N-SIM S Super Resolution Microscope (Nikon, Tokyo, Japan) equipped with 405 nm, 488 nm, 561 nm, and 640 nm excitation light laser combiner (Coherent, Santa Clara, CA, USA), with CFI SR HP Apochromat TIRF 100XC Oil lens. The captured images were reconstructed with NIS-Elements AR (Advanced Research) Imaging Software (Nikon, Tokyo, Japan).
Zhu T., Zhang Y., Sheng X., Zhang X., Chen Y., Zhu H., Guo Y., Qi Y., Zhao Y., Zhou Q., Chen X., Guo X, & Zhao C. (2023). Absence of CEP78 causes photoreceptor and sperm flagella impairments in mice and a human individual. eLife, 12, e76157.
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