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K4bapta

Manufactured by Santa Cruz Biotechnology
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Sourced in United States

K4BAPTA is a calcium chelator used in biochemical research applications. It is a tetra-potassium salt of the compound BAPTA, which is a highly selective and sensitive calcium-binding agent. K4BAPTA's primary function is to effectively capture and control free calcium ions in experimental systems.

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Lab products found in correlation

Chromanol 293b, by Bio-Techne (1 mentions) E 4031, by Bio-Techne (1 mentions) Brain phosphatidylinositol 4 5 bisphosphate pip2, by Avanti Polar Lipids (1 mentions)

2 protocols using k4bapta

1

Preparation of Agonist Stock Solutions

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Ado and ACh (Sigma-Aldrich, St Louis, MO, USA) were dissolved in deionized water to make 10 mM stock solutions and stored at -20°C. Working agonist concentrations were freshly prepared in the recording extracellular solution. E-4031 and chromanol 293B were obtained from Tocris Bioscience (Ellisville, MO, USA), K4BAPTA was acquired from Santa Cruz Technology (Dallas, TX, USA), and all other reagents were from Sigma-Aldrich.
López-Serrano A.L., Zamora-Cárdenas R., Aréchiga-Figueroa I.A., Salazar-Fajardo P.D., Ferrer T., Alamilla J., Sánchez-Chapula J.A., Navarro-Polanco R.A, & Moreno-Galindo E.G. (2022). Differential voltage-dependent modulation of the ACh-gated K+ current by adenosine and acetylcholine. PLoS ONE, 17(1), e0261960.
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2

Preparation of PIP2 and SPM solutions

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SPM was obtained from Sigma-Aldrich, dissolved in water as a 100-mM stock solution, and diluted in the bath solution just before use. Brain phosphatidylinositol 4,5-bisphosphate (PIP2; dissolved in chloroform:methanol:water) was purchased from Avanti Polar Lipids, aliquoted, dried under a nitrogen stream, and stored at −80°C. Before the experiment, PIP2 was dissolved in FVPP solution by sonication for 10 min to obtain a concentration of 10 µM. All other reagents were purchased from Sigma-Aldrich, except K4BAPTA (Santa Cruz Biotechnology).
Marmolejo-Murillo L.G., Aréchiga-Figueroa I.A., Moreno-Galindo E.G., Ferrer T., Zamora-Cárdenas R., Navarro-Polanco R.A., Sánchez-Chapula J.A, & Rodríguez-Menchaca A.A. (2021). Kir4.1/Kir5.1 channels possess strong intrinsic inward rectification determined by a voltage-dependent K+-flux gating mechanism. The Journal of General Physiology, 153(5), e201912540.
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