Anti gata 3 d13c9

Cells were lysed in RIPA buffer, and whole-cell extracts were quantified by the Bradford assay (Bio-Rad). For assessment of nuclear proteins, nuclear extracts were obtained using NE-PER nuclear and cytoplasmic extraction kit (Thermo Scientific). The protein samples or cell lysates were analyzed by SDS-PAGE and Western blot. Briefly, after proteins were transferred onto PVDF membranes (Millipore), the membranes were incubated with indicated primary antibodies, followed by a HRP-conjugated secondary antibody. Immunoreactive bands were detected using the Western Lighting Plus-ECL system (PerkinElmer) or the SuperSignal West Dura Extended Duration Substrate (Pierce). The primary antibodies used for Western blot included anti-p38 (2F11, Millipore), anti-phospho p38 (Thr180/Tyr182) (2BB10, Cell Signaling), and anti-β-actin (C4, Millipore), anti-NKG2A (Abnova), anti-GATA-3 (D13C9, Cell Signaling), anti-phospho-GATA-3 (Ser308) (EPR18118, Abcam), and anti-Histone H3 (BioLegend).