Cocl2 c8661

Recombinant β-type NRG1 was purchased from ProSpec (East Brunswick, NJ, USA). Antibodies were obtained from Millipore Corporation (Chemicon, MA, USA) (EAAT3 (EAAC1), MAB1587), Santa Cruz Biotechnology Inc. (Santa Cruz, CA, USA) (Bcl-X_L, sc-8392; p53, sc-126; β-actin, sc-47778), Novus Biologicals (Centennial, CO, USA) (HIF-1α, NB100-131; Tau, NBP-25613), ThermoFisher scientific (Waltham, MA, USA) (Phospho-Tau (AT8), #MN1020), Mybiosoure (San Diego, CA, USA) (Phospho-Tau (ser422), #A11008), and Cell Signaling Technology (CST, MA, USA) (Caspase 3, #9662 s; cleaved caspase 3, # 9661 s; EAAC1, #12,179; Myc-tag, #2276; HRP-conjugated anti-rabbit IgG, #7076 s; HRP-conjugated anti-mouse IgG, #7074 s). CoCl₂ (C8661) was purchased from Sigma-Aldrich (St. Louis, MO, USA).

MCF-7 cell line was purchased from ATCC ATCC. RPMI 1640 + GlutaMAX^TM, Fetal Bow Serum (FBS), Penicillin/Streptomycin (Gibco- 151140122) and insulin (Gibco 12585-014) were bought from Gibco, Life Technolgies. Immunostaining reagents; HIF-1 α antibody, 2-(4-amidinophenyl)-1H -indole-6-carboxamidine (DAPI, D21490) was purchased from Invitrogen, Alexafluor 488 were provided by histology core facility in Life Sciences department of EPFL. Cobalt chloride, CoCl₂ (C8661) and other chemicals were purchased from Sigma. Gold screen-printed electrodes (Au SPE) used throughout the study were bought from Dropsens.