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Lumplanfln 60x objective

Manufactured by Olympus

The LUMPLanFLN 60x objective is a high-magnification optical lens designed for use with Olympus microscopes. It provides a 60x magnification factor, enabling detailed observation and analysis of microscopic specimens.

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2 protocols using lumplanfln 60x objective

1

Optical Imaging of Tissue Sections

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All tissue sections were imaged on an Olympus FV1000MPE microscope using either an Olympus UPlan SApo 20x objective (NA 0.75) or an Olympus LUMPLanFLN 60x objective (NA 1.0).
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2

In-vivo Raman Mapping of Coral Polyp

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A colony of O. macrospiculata was transferred from Eilat, alive in seawater, to the Weizmann Institute in Rehovot, Israel without any pretreatment. One polyp was excised from the colony and measurements were conducted on various places along the stem of its body with a water immersion 1.0 numerical aperture LUMPlanFL N 60x objective (Olympus) in the original seawater in which the colony was collected, as described previously by [56 ], without anesthetization by MgCl2 addition. Initially, spot analyses were conducted on the polyp so as to stress the animal as little as possible. Spot measurements were repeated after 24 hours on the same colony, including on tissue locations in which no sclerites were observed in order to determine the background signal. At that time, we also conducted an in-vivo Raman mapping of mineral distribution. Map pixels were smoothed in ImageJ.
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