Pro-inflammatory stimulation of tendon-like constructs was done after 7 days of contraction with 10 ng/mL recombinant rat interleukin-1β (rIl-1β; PeproTech, Vienna, Austria) in complete culture medium for 24 h. One hour before PEMF treatment/exposure rIl-1β containing medium was replaced with complete culture medium w/o rIl-1β. Supernatants were collected before rIl-1β treatment (supernatant 1), after 24 h of rIl-1β incubation (supernatant 2) and after the completed PEMF exposure protocol (2 cycles of 60 min PEMF exposure and 90 min resting time each; supernatant 3) and stored at −80 °C until further use.
Ril 1β
Manufactured by Thermo Fisher Scientific
Sourced in Austria, United States
The RIl-1β is a laboratory equipment product designed for the analysis and measurement of interleukin-1 beta (IL-1β) levels. It functions as a tool for researchers and scientists to quantify this key cytokine involved in inflammatory processes. The product specifications and technical details are provided without interpretation or extrapolation on its intended use.
Automatically generated - may contain errors
Lab products found in correlation
Il 23, by Thermo Fisher Scientific (2 mentions)
Primary mouse keratinocytes, by CELLnTEC (2 mentions)
Ril 23, by Thermo Fisher Scientific (2 mentions)
Neutralizing mabs for il 1β, by Thermo Fisher Scientific (2 mentions)
Il 1β, by CELLnTEC (2 mentions)
Mirvana mirna mimic, by Thermo Fisher Scientific (1 mentions)
Tpca 1, by Selleck Chemicals (1 mentions)
5 protocols using ril 1β
1
Tendon-Like Construct Inflammatory Response
2
Skin Cell Isolation and Cytokine Stimulation for Th17 Analysis
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Whole skin cells were prepared from mouse back skin 4 (link). In brief, skin tissues were digested with a buffer containing collagenase IV, hyaluronidase, and DNase I. Skin cells were labeled with CFSE and stimulated with rIL- 23 (1ng/ml, eBioscience), rIL-1β (1ng/ml, eBioscience), or rIL-23 plus rIL-1β for 3 days. Cell proliferation and intracellular IL-17 were measured by flow cytometry. For blocking experiment, neutralizing mAbs for IL-1β (2μg/ml, eBioscience), IL-23 (12.5μg/ml, eBioscience), or matched isotype control mAbs were added. In addition, primary mouse keratinocytes (Cellntec) were stimulated with IL-1β for 6 hours and RNA were extracted for analysis of chemokine expression by real-time qPCR. For γδT cell development study, skin, thymus and BM were taken from pups before (E18 or E20) or after birth.
3
Skin Cell Isolation and Cytokine Stimulation for Th17 Analysis
4
IL-1β and IL-6 Modulate PD-L1 in Osteosarcoma
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To determine the effects of IL-1β and IL-6 on PD-L1 expression, human osteosarcoma cells were seeded in 6-well plates at a density of 1 × 105 cells/well. Next, the cells were treated with 10 ng/mL of recombinant human IL-1β (rIL-1β; PeproTech, Cranbury, NJ, USA) or 20 ng/mL of recombinant IL-6 (rIL-6; StemCellTM Technologies, Vancouver, BC, Canada). After 24 h, the cell lysates were collected for qRT-PCR. For flow cytometry analysis, the osteosarcoma cells were treated with recombinant human IL-1β or IL-6 for 48 h and analyzed for cell surface PD-L1 expression.
5
Modulation of Astrocyte Inflammatory Response
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Cells were transfected with mimic pre-miRNA for miR146a or miR147b (mirVana miRNA mimics, Applied Biosystems, Carlsbad, CA, USA) for 24 hours as described previously (van Scheppingen et al. 2016b) .
Astrocyte cultures were stimulated with human recombinant (r)IL-1 β (10 ng/ml; Peprotech, Rocky Hill, NJ, USA) or lipopolysaccharide (LPS; 100 ng/ml; Sigma, St. Louis, MO, USA) for 24 hours. Viability of human cell cultures was not influenced by the treatments (as shown previously; (van Scheppingen et al. 2016a )). To examine the effect of the IκB kinase-2 (IKK-2) inhibitor TPCA-1 in astrocyte cultures, treatment with 1 or 5 µM TPCA-1 (Selleck Chemicals, Munich, Germany) in DMSO (0.05% final DMSO concentration) was started 1 hour before stimulation with IL-1β was initialized, and treatment was continued during stimulation.
Astrocyte cultures were stimulated with human recombinant (r)IL-1 β (10 ng/ml; Peprotech, Rocky Hill, NJ, USA) or lipopolysaccharide (LPS; 100 ng/ml; Sigma, St. Louis, MO, USA) for 24 hours. Viability of human cell cultures was not influenced by the treatments (as shown previously; (van Scheppingen et al. 2016a )). To examine the effect of the IκB kinase-2 (IKK-2) inhibitor TPCA-1 in astrocyte cultures, treatment with 1 or 5 µM TPCA-1 (Selleck Chemicals, Munich, Germany) in DMSO (0.05% final DMSO concentration) was started 1 hour before stimulation with IL-1β was initialized, and treatment was continued during stimulation.
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