Orca hr c4742 95 12hr

Manufactured by Hamamatsu Photonics

The ORCA HR (C4742-95-12HR) is a high-resolution, scientific-grade CCD camera designed for a variety of imaging applications. It features a 1344 x 1024 pixel CCD sensor with a large active area, enabling the capture of high-quality images. The camera provides a fast readout speed and high quantum efficiency, making it suitable for low-light imaging tasks.

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Lab products found in correlation

P36935, by Thermo Fisher Scientific (1 mentions)

Close spelling variants of this product

Orca C4742-95 C4742-95 ORCA-HR C4742

3 protocols using orca hr c4742 95 12hr

Cytospin Preparation and Immunostaining

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For preparation of cytospins from both cultures, a volume of 100 - 200 µL containing a number of 5 × 10⁴ to 2 × 10⁵ viable cells was added in a pre-assembled cytospin buckets including frosted slides (SLS, MIC3806).Then the buckets were spun at 60 × 10 for 5 min with the acceleration off in a specific cytospin machine (Shandon). Buckets were dismantled, and the slides left to dry at RT overnight. Lastly, the slides were wrapped in a foil and freezed at -8 °C. The cytospin quality was assessed by mounting the cytospin in a drop of reagent with DAPI (Invitrogen, P36935), then viewed under the immunofluorescence microscope.
We followed the manufacturer recommended protocols for immunostaining of cytospins for NPM. After which, the slides were viewed, with the Nikon Eclipse 80I fluorescence microscope (ICCT technologies, Canada), equipped with three fluorescence filter cubes. The obtained images were grabbed with the Hamamatsu ORCA HR (C4742-95-12HR) high resolution digital camera with full remote control from PC. Fast high sensitivity black and white camera was used for low intensity fluorescence slides. Analysis of the obtained images was conducted with the NIS-elements software.

Khaled S.A., Burthem J., Abo Elnoor E.B., ElToni L.F., Ahmed H.M, & Ahmed S.M. (2017). Role of Nucleophosmin Gene Mutation in Leukemogenesis of Acute Myeloid Leukemia. Journal of Hematology, 7(1), 7-13.

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Structural Characterization of Transcribed RNAs

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RNAs were transcribed using AmpliScribe T7 High Yield Transcription Kit. Transcribed RNAs were gel purified (8% urea polyacrylamide gel). Purified RNA samples were supplemented with 10 mM sodium cacodylate pH 6.8, then heated to 90 degrees C for 30 seconds and slowly cooled to room temperature. The annealed RNA samples were incubated with 10 mM MgCl₂ for 20 min. 2 μl of 200 ng/μl RNA sample was applied to glow discharged carbon-coated grids. Grids were stained with 2% uranyl acetate. The EM micrographs were collected on a Tecnai G² Spirit BioTWIN with Hamamatsu ORCA-HR C4742-95-12HR detector at magnification of 49000X. Image processing and particle picking was performed using EMAN2 (Tang et al., 2007). 500 particles were included for all analysis. Scikit-image was used to measure the diameter and circularity of particles. The results were then plotted using matplotlib.

Du P., Wang L., Sliz P, & Gregory R.I. (2015). A biogenesis step upstream of Microprocessor controls miR-17~92 expression. Cell, 162(4), 885-899.

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Immunocytochemistry Protocol for NPM

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Firstly cellular cytospins were prepared from living cells. Then, we followed the manufacturer recommended protocols for immunostaining of cytospins for wtNPM and pan -NPM. Next, the slides were viewed, with the Nikon Eclipse 80I fluorescence microscope (ICCT Technologies, Canada), equipped with three fluorescence filter cubes. The obtained images were grabbed with the Hamamatsu ORCA HR (C4742-95-12HR) high resolution digital camera, with full remote control from PC. Another fast high sensitivity black and white camera was used for low intensity fluorescence slides. Immunofluorescence images were captured, analyzed and saved with the NIS-Elements imaging software. Also, creation of overlay images (e.g. 4',6-diamidino-2-phenylindole (DAPI)/NPM overlay) was carried out with the same program. The saved images were edited with the Adobe Photoshop or Picasa programs.

Khaled S.A., Burthem J., Elnoor E.B., ElToni L.F., Ahmed H.M, & Ahmed S.M. (2019). Quantitative Assay of Mutated Nucleophosmin in Acute Myeloid Leukemia. Journal of Hematology, 8(3), 111-120.

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