Dimethyl sulfoxide (DMSO) was obtained from Amresco Incorporation (USA); 2-chloro-N (6)-cyclopentyl-adenosine (CCPA), 8-cyclopentyl-1,3-dipropylxanthine (DPCPX), quick-hardening mounting medium, and mouse anti-β-tubulin antibody (Cat. T8328) were purchased from Sigma Corporation (USA); Hematoxylin and Eosin (HE) staining kit was obtained from Beyotime Institute of Biotechnology (China); 3,3-diaminobenzidine (DAB) was purchased from ZSGB-BIO Corporation (China); Bicinchoninic acid (BCA) protein assay Kit and ECL western blotting detection reagent were purchased from Pierce Corporation (USA); TUNEL assay kit was from Roche Limited (USA); PCR primer was obtained from Invitrogen Company (USA); Rabbit anti-caspase-3 antibody (Cat. 9662), HRP-labeled goat anti-rabbit secondary antibody (Cat. 7074), and HRP-labeled horse anti-mouse secondary antibody (Cat. 7076) were purchased from Cell Signal Technology (USA); Rabbit anti-Syntaxin (Cat. ab188583), rabbit anti-PKC (Cat. ab19031), rabbit anti-PKC-ζ (Cat. ab59364), and rabbit anti-Gα (i; Cat. ab58916) antibodies were obtained from Abcam Company (USA). Mouse anti-β-tubulin antibody was purchased from Beyotime Biotechnology (China).
Rabbit anti pkc
Manufactured by Abcam
Sourced in United States, United Kingdom
Rabbit anti-PKC is a primary antibody that recognizes protein kinase C (PKC), a family of serine/threonine-specific protein kinases involved in various cellular processes. This antibody can be used for the detection and analysis of PKC in research applications.
Automatically generated - may contain errors
2 protocols using rabbit anti pkc
1
DMSO-Mediated Neuroprotection Analysis
2
Protein Extraction and Western Blotting
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Total protein was extracted from RPE cells using radioimmunoprecipitation assay (RIPA) buffer supplemented with phenylmethylsulfonyl fluoride (PMSF) at the ratio of 100:1, and quantified using the bicinchoninic acid (BCA) assay. Equal amounts of protein per sample were separated through 10% to 15% sodium dodecyl sulfate polyacrylamide (SDS-PA) gel, and the bands were transferred onto polyvinylidene difluoride (PVDF) membranes according to the manufacturer's protocol. After blocking with a commercially available blocking buffer (Beyotime, Shanghai, China), the blots were incubated overnight with primary antibodies (rabbit anti-TRPM7, rabbit anti-PKC, rabbit anti-ERK, and rabbit anti-GAPDH primary antibodies, Abcam, UK) at 4°C.
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