Thiazolyl blue tetrazolium bromide mtt

Manufactured by Bio Basic

Sourced in Canada

Thiazolyl blue tetrazolium bromide (MTT) is a colorimetric assay used to measure the metabolic activity of cells. It is a yellow tetrazolium salt that is reduced to purple formazan crystals in living cells, indicating the presence of metabolically active cells.

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Lab products found in correlation

Dimethyl sulfoxide (dmso), by Merck Group (2 mentions) Lamin a c, by Santa Cruz Biotechnology (1 mentions) Gapdh, by Santa Cruz Biotechnology (1 mentions) Bcl 2, by Santa Cruz Biotechnology (1 mentions) Cetyltrimethylammonium bromide, by Bio Basic (1 mentions) Phosphate buffered saline tablet, by Merck Group (1 mentions) Hydrogen tetrachloroaurate iii haucl4 3h2o, by Merck Group (1 mentions) Sodium borohydride nabh4, by Merck Group (1 mentions) Dulbecco s modified eagle medium dmem, by Nacalai Tesque (1 mentions) Triclosan, by Merck Group (1 mentions) Pdadmac, by Merck Group (1 mentions) Mannitol, by Merck Group (1 mentions) Horseradish peroxidase hrp substrate, by Merck Group (1 mentions) M pertm mammalian protein extraction reagent, by Thermo Fisher Scientific (1 mentions) Apoptosis detection kit, by Elabscience (1 mentions) Dulbecco s modified eagle s medium dmem, by Nacalai Tesque (1 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions) Fetal bovine serum (fbs), by GE Healthcare (1 mentions) Taq dna polymerase, by New England Biolabs (1 mentions) Revertaid m mulv reverse transcriptase, by Thermo Fisher Scientific (1 mentions)

4 protocols using thiazolyl blue tetrazolium bromide mtt

Comprehensive Immunoblotting and Viability Assay

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For immunoblotting and immunocytochemistry, primary antibodies for emerin (#sc-15378, 1:4500), Lamin A/C (#sc-20681, 1:4500), BAF (#sc-166324, 1:5000), Bcl2 (#sc-7382, 1:4500), and GAPDH (#sc-47724, 1:5500) were purchased from Santa Cruz (Santa Cruz Biotechnology, Santa Cruz, CA, USA). Primary antibodies specific for pSTAT3 (#ab76315, 1:3000) were purchased from Abcam (Cambridge, U.K.). Primary antibodies specific for HA (1:5000), Flag (1:5000), and β-actin (1:5000) were purchased from MBL (Woburn, MA, USA). Primary antibodies specific for STAT3 (1:3000) were purchased from Cell Signaling (Danvers, MA, USA). Primary antibodies for Myosin Heavy Chain (MYHC, #MAB4470, 1:50) were purchased from R&D systems (Minneapolis, MN, USA). For the viability assay, cells were treated H₂O₂ for cell death, and thiazolyl blue tetrazolium bromide (MTT) was purchased from Bio Basic (Markham, ON, Canada).

Lee B., Lee S., Lee Y., Park Y, & Shim J. (2021). Emerin Represses STAT3 Signaling through Nuclear Membrane-Based Spatial Control. International Journal of Molecular Sciences, 22(13), 6669.

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Synthesis of Gold Nanoparticles

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Ascorbic acids, thiazolyl blue tetrazolium bromide (MTT) and cetyltrimethylammonium bromide (CTAB), were purchased from BioBasic (Canada). Hydrogen tetrachloroaurate(III) (HAuCl₄·3H₂O) and phosphate-buffered saline (PBS) tablets were purchased from Sigma-Aldrich (Germany). Dimethyl sulfoxide (DMSO) and sodium borohydride (NaBH₄) were purchased from Merck (Germany). Sodium alginate (300–400 mPa·s) was obtained from Chemiz (Malaysia). Triclosan, and poly(diallyldimethylammonium chloride) PDADMAC (20 wt. % in H₂O) were obtained from Sigma-Aldrich (USA). Muller Hinton Broth (MHB) was purchased from BD Difco™ (USA). Glucose (1%) was obtained from Systerm (Malaysia). Silver nitrate (AgNO₃) was purchased from Bendosen (Malaysia). Dulbecco’s modified Eagle medium (DMEM) was obtained from Nacalai Tesque (Kyoto, Japan). Unless otherwise specified, all the reagents were prepared using Milli-Q ultrapure water.

Manimaran M., Teo Y.Y., Kah J.C., Beishenaliev A., Loke Y.L., Foo Y.Y., Ng S.F., Chee C.F., Chin S.P., Faruqu F.N., Chang C.Y., Misran M., Chung L.Y., Leo B.F., Chiou S.H., Chang C.C., Tay S.T, & Kiew L.V. (2024). PDADMAC/Alginate-Coated Gold Nanorod For Eradication of Staphylococcus Aureus Biofilms. International Journal of Nanomedicine, 19, 3697-3714.

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Alginate-Based Nanoparticle Synthesis and Characterization

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Sodium alginate (300 – 400 mPa⋅s) was purchased from Chemiz (Malaysia). Gold (III) chloride trihydrate (HAuCl₄·3H₂O), 3,3′-anthracene-9,10-diyldipropanoic acid (ADPA), 1,3-diphenylisobenzofuran (DPBF), triton X-100 and mannitol were purchased from Sigma-Aldrich (Germany). Sodium borohydride (NaBH₄), dimethyl sulfoxide (DMSO) and horseradish peroxidase (HRP) substrate were supplied by Merck (Germany). M-PER^TM mammalian protein extraction reagent was purchased from Thermo Fischer Scientific (USA). Thiazolyl blue tetrazolium bromide (MTT) and ascorbic acid were obtained from BioBasic (Canada). Hexadecyltrimethylammonium bromide (CTAB) was purchased from Sangon Biotech (China). Silver nitrate (AgNO₃) was acquired from Bendosen (Malaysia). Dulbecco’s modified Eagle’s medium (DMEM) was purchased from Nacalai Tesque (Japan). Fetal bovine serum (FBS) was purchased from GIBCO™ (USA). Apoptosis detection kit was supplied by Elabscience (USA). Unless stated otherwise, all reagents were prepared using Milli-Q ultrapure water.

Loke Y.L., Beishenaliev A., Wang P.W., Lin C.Y., Chang C.Y., Foo Y.Y., Faruqu F.N., Leo B.F., Misran M., Chung L.Y., Shieh D.B., Kiew L.V., Chang C.C, & Teo Y.Y. (2023). ROS-generating alginate-coated gold nanorods as biocompatible nanosonosensitisers for effective sonodynamic therapy of cancer. Ultrasonics Sonochemistry, 96, 106437.

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Evaluating Cytokine and Cell Viability

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Dimethyl sulfoxide (DMSO) was purchased from Sigma-Aldrich (St. Louis, MO, USA). Thiazolyl blue tetrazolium bromide (MTT) was obtained from Bio Basic (Markham, ON, Canada). Fetal bovine serum (FBS), RPMI, antibiotics and antimycotic agents were procured from GE Healthcare (Chicago, IL, USA). The human IL-8 ELISA kit was purchased from Abcam (Cambridge, UK). The GENEzol reagent kit was obtained from Geneaid Biotech (New Taipei City, Taiwan). RevertAid M-MuLV reverse transcriptase was purchased from Thermo Fisher Scientific (Waltham, MA, USA), and Taq DNA Polymerase was obtained from New England Biolabs (Ipswich, MA, USA).

Nemidkanam V., Kato Y., Kubota T, & Chaichanawongsaroj N. (2020). Ethyl acetate extract of Kaempferia parviflora inhibits Helicobacter pylori-associated mammalian cell inflammation by regulating proinflammatory cytokine expression and leukocyte chemotaxis. BMC Complementary Medicine and Therapies, 20, 124.

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