Xbridge beh c18 xp column

Manufactured by Waters Corporation

Sourced in United States

The XBridge BEH C18 XP Column is a high-performance liquid chromatography (HPLC) column designed for reversed-phase separations. It features a bonded C18 stationary phase and bridge-ethyl hybrid (BEH) particle technology. The column is intended for a wide range of analytical applications requiring high-resolution, reproducible separations.

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Lab products found in correlation

Tandem mass tag (tmt), by Thermo Fisher Scientific (1 mentions) Q exactive hfx orbitrap instrument, by Thermo Fisher Scientific (1 mentions) Acquity uplc system, by Waters Corporation (1 mentions) Xevo tq ms mass spectrometer, by Waters Corporation (1 mentions) Targetlynx xs software, by Waters Corporation (1 mentions) Oasis hlb solid phase extraction cartridge, by Waters Corporation (1 mentions) Lc 20adxr, by Shimadzu (1 mentions) Whatman, by Cytiva (1 mentions)

Spelling variants of this product

BEH C18 column (542 citations) XBridge C18 (505 citations) XBridge C18 column (428 citations) C18 column (384 citations) BEH C18 (162 citations) XBridge BEH C18 column (87 citations) XBridge BEH C18 (36 citations) XBridge BEH C18 XP (6 citations) XBridge C18 XP column (2 citations) XBridge Columns (2 citations)

5 protocols using xbridge beh c18 xp column

Quantitative Proteomics by TMT Labeling

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The digested samples were added with 15 μl of TMT (Thermo Scientific, United States) and incubated at room temperature for 1 h, then added with 2 μl of 5% hydroxylamine and incubated again at room temperature for 15 min. The collected fractions were SDC cleanup with 2% trifluoroacetic acid (TFA, Sigma, United States) and peptide desalting with desalting buffer work solution Acetonitrile (ACN, ANPEL Laboratory Technologies, China). The samples were performed in XBridge BEH C18 XP Column (Waters, United States).
For nanoLCMS/MS analysis, 1 μg of total peptides for each sample were conducted on a nano-UPLC (EASYnLC1200) coupled to a Q Exactive HFX Orbitrap instrument (Thermo Fisher Scientific). A reversed-phase column (100 μm ID ×15 cm, Reprosil-Pur 120 C18-AQ, 1.9 μm, Dr. Math) was used. Mobile phases A were H₂O with 0.1% formic acid (FA, Sigma, United States), 2% ACN, and phase B were 80% ACN, 0.1% FA. The samples were separated with a gradient of 90 min at a flow rate of 300 nL/min in gradient A, while gradient B: 2–5% for 2 min, 5–22% for 68 min, 22–45% for 16 min, 45–95% for 2 min, 95% for 2 min. Finally, data-dependent acquisition (DDA) was performed in profile and positive mode with an Orbitrap analyzer.

Chen Z., Chen Z., Zhu J., He J., Liu Q., Zhu H., Lei A, & Wang J. (2022). Proteomic Responses of Dark-Adapted Euglena gracilis and Bleached Mutant Against Light Stimuli. Frontiers in Bioengineering and Biotechnology, 10, 843414.

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Quantification of Short-Chain Fatty Acids

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The method of quantification of AA, PA, and BA in the fecal suspension by ULPC and diode array detection (DAD) (Agilent) was validated. A detailed description can be found in the Supplementary Materials (S1; S2 and Tables S1 and S2). In brief, liquid–liquid extraction of the samples was performed. Chromatographic separation was performed on an XBridge BEH C18 XP column (150 mm × 4.6 mm internal diameter (i.d.)) with a particle size of 2.5 μm (Waters, Milford, MA, USA) protected by a guard column of the same type (5 mm × 3.9 mm i.d.). The DAD detector was set at the wavelength of 210 nm using the peak width of 5 Hz. Data processing was performed using Open Lab CDS ChemStation Edition for LC & LC/MS Systems Rev C.01.07.SR2 [255] (Agilent Technologies, Santa Clara, CA, USA). Levels of SCFAs were normalized for 1 g of feces.

Steenbeke M., Valkenburg S., Gryp T., Van Biesen W., Delanghe J.R., Speeckaert M.M, & Glorieux G. (2021). Gut Microbiota and Their Derived Metabolites, a Search for Potential Targets to Limit Accumulation of Protein-Bound Uremic Toxins in Chronic Kidney Disease. Toxins, 13(11), 809.

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High pH Fractionation of Polypeptides

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Polypeptide samples (100 μg) were carried out by High pH reverse phase fractionation (pH = 10). Chromatographic column: XBridge BEH C 18 XP Column (150 × 2.1 mm, Waters). The mobile phase A was ammonium formate (AF) water solution: 10 mM, pH = 10. Mobile phase B: 10 mM AF, 10% H₂O, 90% ACN, pH = 10. Samples were divided into 120 min gradient intervals. Gradient B: 5–28% for 78 min, 28–50% for 12 min, 50–80% for 2 min, 80% for 4 min, 80–5% for 2 min, 5% for 20 min. The polypeptide was separated into 180 parts and collected in 40 s intervals. Lastly, all samples were merged into 20 components and then were vacuum dried and stored at –80°C before liquid chromatography-mass spectrometry (LC-MS)/MS analysis.

Luo W., Yang Z., Zhang W., Zhou D., Guo X., Wang S., He F, & Wang Y. (2022). Quantitative Proteomics Reveals the Dynamic Pathophysiology Across Different Stages in a Rat Model of Severe Traumatic Brain Injury. Frontiers in Molecular Neuroscience, 14, 785938.

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UPLC-MS/MS Analysis of Mycotoxins

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UPLC analysis was performed on a Waters Acquity UPLC system (Waters, Milford, MA, USA). Separation was achieved on a Waters XBridge® BEH-C₁₈ XP column (130 Å, 2.5 µm, 3.0 × 100 mm, PN: 186006035) at 40°C. The mobile phase consisted of (A) acetonitrile and (B) water containing 5 mmol/L ammonium acetate, and a linear gradient elution program was applied as follows: initial, 10% A; 1 min, 10% A; 3 min, 70% A; 5 min, 90% A; 6 min, 90% A; 6.1 min, 10%; 8 min, 10% A. The mobile phase flow rate was 0.4 ml/min.
The separated compounds were analyzed by a Waters XEVO TQMS mass spectrometer (Waters, Milford, MA, USA) with an electrospray ionization source operated in negative mode (ESI⁻) for ZEN and ALS, and in positive mode (ESI⁺) for the other mycotoxins. Multiple reaction monitoring (MRM) mode was established as shown in Supplementary Table 2. The source parameters are set as follows: capillary voltage of 2.5 kV for ESI⁺ and 1.5 kV for ESI⁻, ion source temperature of 150°C, and desolvation temperature of 500°C. The gas flow rates were 7.0 bar for nebulizing gas and 1,000 L/h for desolvation gas, respectively. TargetLynx XS software was used to process the data (Waters Corporation, Milford, MA, USA).

Meng J., Li R., Huang Q., Guo D., Fan K., Zhang J., Zhu X., Wang M., Chen X., Nie D., Cao C., Zhao Z, & Han Z. (2023). Survey and toxigenic abilities of Aspergillus, Fusarium, and Alternaria fungi from wheat and paddy grains in Shanghai, China. Frontiers in Plant Science, 14, 1202738.

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Water sample extraction and chemical analysis

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The method of the sample pretreatment and extraction mainly referred to previous studies (Chen et al., 2016a; Chen et al., 2017) . In short, the water sample (1 L) was filtered through 0.7 μm glass microfiber filters to remove suspended particles and then spiked with mixed ISs (100 ng each IS). Filtered samples were extracted by the reversed-phase Oasis HLB solid-phase extraction (SPE) cartridge (200 mg, 6 mL, Waters Corporation, Milford, MA, USA). The final extract was concentrated to 1 mL, followed by syringe filtration (0.22 μm, PTFE, Whatman, USA) and transferred to 2 mL amber vials for storage at -18 °C before analysis. Triplicate samples were conducted for each sampling site. Detailed information on reagents and pretreatment is in SI. The UPLC-ESI-MS/MS (ultra-high-performance liquid chromatography (LC-20ADXR, Shimadzu, Kyoto, Japan) -electrospray ionization tandem mass spectrometer (AB Sciex API 4500, Applied Biosystems, Foster City, CA, USA)) was employed to detect the target chemicals in water samples. It was performed in the negative multiple reaction monitoring (MRM) model with unit mass resolution. The LC system was equipped with a Waters Xbridge BEH-C18 XP column (particle size 2.5 µm, 2.1 mm × 100 mm), which had a pre-column (2.5 µm, 2.1 mm × 5 mm) for chemical separation. The detailed instrumental setting for chemical detection is in SI Table S3 and Fig. S1.

Lei K., Zhu Y., Chen W., Pan H.Y., Guo B.B., Zhang X., Cao Y.X., Sweetman A.J, & Lin C.Y. (2018). The occurrence of home and personal care products in the Haihe River catchment and estimation of human exposure. The Science of the total environment, 643.

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