Minispec mq60 60 mhz td nmr broadband spectrometer

Equal amounts of PtCo@G were added into H₂O, PBS (pH 1.0), and SGF and incubated for 12 h. Then the digital photos, hydrodynamic diameter, MR T₂ relaxivity and T₂-weighted phantom images of PtCo@G in H₂O, PBS (pH 1.0), and SGF were measured by DLS (Malvern, UK), Minispec MQ60 60 MHz TD NMR broadband spectrometer (Bruker, Germany) and 1.5 T small animal MR scanner (MRI 1.5 T; Shining Global Science and Education Equipment Co., Ltd., Shanghai, China), respectively.

TEM images were taken with a JEM-2010 (JEOL, Japan). Ultraviolet–visible spectra were recorded by a UV-2450 spectrophotometer (Shimadzu, Japan). Hydrodynamic diameters and ζ-potentials were measured by a DLS system (Malvern, UK). Cell viability and urease activity assays were measured by a multi-mode microplate reader (Bio-Tek, USA). A Raman imaging microscope system was used to record the Raman spectra with 633 nm laser excitation (Renishaw, UK). A Minispec MQ60 60 MHz TD NMR broadband spectrometer (Bruker, Germany) was used to measure T₂ relaxivity. T₂-weighted MR imaging was taken by the 1.5 T small animal MR scanner (Shinning Globe MRI 1.5 T, China). Gram-stained slices were observed using a BX 51 microscope (Olympus, Japan). Confocal laser scanning microscopy was performed using an FV 1000 laser confocal microscope (Olympus, Japan).

All Raman measurements were performed in a Renishaw’s InVia Raman system with 633 nm laser excitation (Renishaw, UK). Ultraviolet-visible spectra were recorded by a UV-2450 UV-vis spectrophotometer (Shimadzu, Japan). Hydrodynamic diameters and Zeta potentials were measured by a DLS system (Malvern, UK). TEM images were taken with a JEM-2010 (JEOL, Japan). SEM images were measured by JSM-6700F (Japan). Fluorescence spectra were performed by HORIBA Fluoromax-4 (HORIBA, USA). Electron paramagnetic resonance (EPR) measurements were recorded by JES-FA200 electron spin resonance (JEOL, Japan). A Minispec MQ60 60 MHz TD NMR broadband spectrometer (Bruker, Germany) was used to measure T₂ relaxivity. T₂-weighted MR imaging was taken by the 1.5 T small animal MR scanner (MRI 1.5 T; Shining Global Science and Education Equipment Co., Ltd., Shanghai, China). Inductively coupled plasma mass spectrometry (ICP-MS, Agilent, USA) was utilized to measure the concentrations of released Co²⁺ and Pt²⁺ in the solution.