Geraniin (purity ≥ 95%) was isolated and purified from leaves of Phyllanthus muellerianus [2 (link)]. Urolithin A (purity ≥ 98%) was obtained from Santa Cruz (Germany) and dihydrodichlorofluorescein-diacetate (H2DCF-DA) from Invitrogen (Austria). All other chemicals or enzymes were obtained from Sigma-Aldrich (Austria). All primary and secondary antibodies were obtained from New England Biolabs (Germany), except the anti-iNOS and the anti-p65 antibodies from Santa Cruz (Germany), the anti-caspase1 antibody from Abcam (UK), the anti HO-1 antibody from Enzo Life Sciences (Germany) and the FITC-coupled anti-rabbit antibody from Sigma-Aldrich (Austria).
Anti ho 1 antibody
Manufactured by Enzo Life Sciences
Sourced in United States
The Anti-HO-1 Antibody is a laboratory reagent used for the detection and quantification of the HO-1 (Heme Oxygenase-1) protein in various biological samples. HO-1 is an enzyme involved in the breakdown of heme, a vital component of hemoglobin. This antibody can be utilized in techniques such as Western blotting, ELISA, and immunohistochemistry to study the expression and localization of HO-1 in cells and tissues.
Automatically generated - may contain errors
Lab products found in correlation
Anti β actin antibody, by Merck Group (2 mentions)
Anti akt, by Cell Signaling Technology (2 mentions)
Anti caspase 1 antibody, by Abcam (1 mentions)
Urolithin a, by Santa Cruz Biotechnology (1 mentions)
Pvdf blocking reagent for can get signal, by Toyobo (1 mentions)
Anti nrf2 antibody, by Abcam (1 mentions)
Ecl reaction solution, by GE Healthcare (1 mentions)
Cytotoxic detection kit, by Roche (1 mentions)
Horseradish peroxidase conjugated anti rabbit or anti mouse secondary antibodies, by GE Healthcare (1 mentions)
Ripa buffer, by Santa Cruz Biotechnology (1 mentions)
Protein assay kit, by Bio-Rad (1 mentions)
Polyvinylidene fluoride pvdf membrane, by Bio-Rad (1 mentions)
Pierce bca protein analysis kit, by Thermo Fisher Scientific (1 mentions)
Pvdf membrane, by Merck Group (1 mentions)
Anti twist1, by Cell Signaling Technology (1 mentions)
Anti ucp1, by Cell Signaling Technology (1 mentions)
Anti nov ccn3, by Cell Signaling Technology (1 mentions)
Anti sirt1, by Cell Signaling Technology (1 mentions)
Anti sod1, by Cell Signaling Technology (1 mentions)
Anti mmp2, by Cell Signaling Technology (1 mentions)
6 protocols using anti ho 1 antibody
1
Geraniin and Urolithin A Extraction
2
Denazyme AP Protease from Aspergillus oryzae
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Denazyme AP, a protease from Aspergillus oryzae, was supplied from NAGASE Chemtex Co. Ltd. (Osaka, Japan). The commercial rice protein, Oryza Protein-P70, was a gift from Oryza Oil and Fat Chemical Co. Ltd. (Aichi, Japan). Minimum Essential Medium (MEM) was purchased from Sigma-Aldrich (St. Louis, MO). The Cytotoxic Detection Kit was purchased from Roche Diagnostics (Mannheim, Germany). The protein assay kit was purchased from Bio-Rad Laboratories Inc. (Hercules, CA). RIPA buffer was obtained from Santa Cruz Biotechnology (Dallas, TX). The polyvinylidene fluoride (PVDF) membrane was purchased from Bio-Rad (Hercules, CA). The PVDF Blocking Reagent for Can Get Signal® was purchased from Toyobo (Osaka, Japan). Anti-Nrf2 antibody was purchased from Abcam (Cambridge, United Kingdom). Anti-HO-1 antibody was purchased from Enzo Life Sciences (Farmingdale, NY). Anti-β-actin antibody was purchased from Sigma-Aldrich. Horseradish peroxidase-conjugated anti-rabbit or anti-mouse secondary antibodies and ECL reaction solution were obtained from GE Healthcare (Buckinghamshire, UK). Synthetic peptides were purchased from Invitrogen (Thermo Fisher Scientific, Waltham, MA).
3
Protein Extraction and Western Blot Analysis
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Cells were lysed by the protein extraction solution (Sigma-Aldrich Co., St. Louis, MO, United States) and then placed on ice for 20 min before centrifuged 14,000 rpm for 30 min for protein extraction. The supernatants were collected as whole cell protein samples followed by quantitative analysis using the pierce™ BCA protein analysis kit (Thermo Scientific, Waltham, MA). 25 μg proteins were prepared and boiled at 95°C for 5 min and subjected to SDS-PAGE. After transferring protein to the PVDF membrane (Millipore, Billerica, MA) and blocking with 7.5% skim milk in TBST at room temperature for an hour, the membrane was hybridized with anti–HO-1 antibody (Enzo, Farmingdale, NY) overnight at 4°C. After washing with PBST thrice, the membrane was hybridized with anti-rabbit secondary antibody for 1 h. Protein signals were visualized by enhanced chemiluminescence (EMD Millipore, Billerica, MA) using Fujifilm Super RX-N films (Valhalla, NY), and the intensity of signals was analyzed by ImageJ (Schneider et al., 2012 (link)).
4
Western Blot Analysis of Adipose and Liver Proteins
5
Pygenic Acid A Modulates Cellular Signaling
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Materials Pygenic acid A was gifted from NIKOM (National Institute for Korean Medicine Development, 94 Hwarang-ro, Gyeongsan-si, Gyeongsangbuk-do, Korea). Anti-LC3B, anti-p62, anti-PARP, anti-survivin, anti-Livin, anti-XIAP, anti-cIAP1, anti-cIAP2, anti-p21, anti-cyclin D1, anti-FOXM1, anti-phospho STAT3, anti-STAT3, anti-phospho Akt, anti-Akt, anti-phospho p38, anti-p38, anti-phospho eIF2α, anti-eIF2α, anti-IRE1α, anti-GAPDH antibodies were purchased from Cell Signaling Technology (Beverly, MA, USA). Anti-HO-1 antibody, Horseradish peroxidase (HRP)-conjugated rabbit IgG, and HRP-conjugated mouse IgG were purchased from Enzo Life Sciences (Farmingdale, NY, USA). Anti-Vinculin and firefly luciferase, Rabbit IgG, polyclonal-Isotype Control antibodies were purchased from Abcam (Cambridge, UK). Anti-β-actin antibody, Dimethyl sulfoxide (DMSO), and Poly (2-hydroxyethyl methacrylate) (Poly-HEMA) were purchased from Sigma-Aldrich Corporation (St. Louis, MO, USA).
6
Western Blot Analysis of GM-CSF and MDSC Proteins
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Cell lysates of GM-CSF + BM cells and GR-1 + MDSCs (1 × 106) were obtained. After electrophoresis and membrane transfer, antigen–antibody reactions were performed by adding anti-acetyl-histone H4 (Lys8) antibody (#2594, Cell Signaling Technology, Beverly, MA, USA), anti-HO-1 antibody (#ENZ-ABS663, Enzo Life Sciences, Farmingdale, NY, USA), anti-iNOS antibody (#ab3523, Abcam, Cambridge, MA, USA), and anti-β-actin antibody (#NB600-501, Novus Biologicals, Littleton, CO, USA). The specific procedure was as described in literature [20 (link)].
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