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Mouse pdgf

Manufactured by Thermo Fisher Scientific
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Mouse PDGF is a laboratory reagent produced by Thermo Fisher Scientific. It is a recombinant protein that represents the platelet-derived growth factor from mice. The core function of this product is to serve as a research tool for studying cellular processes and signaling pathways involving the platelet-derived growth factor in mouse models.

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Lab products found in correlation

Pen strep, by Thermo Fisher Scientific (1 mentions) Mouse egf, by Thermo Fisher Scientific (1 mentions) Human bfgf, by R&D Systems (1 mentions) Non adherent 24 well plates, by Corning (1 mentions) Mouse oncostatin m, by R&D Systems (1 mentions) Human endothelial medium, by Thermo Fisher Scientific (1 mentions) Non essential amino acids (neaa), by Thermo Fisher Scientific (1 mentions) Dmem f12, by Thermo Fisher Scientific (1 mentions)

Close spelling variants of this product

Mouse PDGF-AA

2 protocols using mouse pdgf

1

Spheroid Formation from Sorted Cells

Cited 3 times
Check if the same lab product or an alternative is used in the 5 most similar protocols
Mouse sorted cells at 1 × 103 were transferred to non-adherent 24 well plates (Corning) with spheroid-forming media5 (link), 7 (link), 47 (link) [1:2 ratio of DMEM F12 (Gibco) and Human Endothelial Medium (Gibco) supplemented with 3.75% Chicken Extract (US Biological), 0.1 mM β-ME (Invitrogen), 1% Non-essential amino acids (Gibco), 1% Pen-strep (Gibco), 1% N2 (Gibco), 2% B27 (Gibco), 20 ng/mL human bFGF (R&D Systems), 20 ng/mL mouse PDGF (Peprotech), 20 ng/mL mouse oncostatin M (R&D Systems), 20 ng/mL mouse IGF-1 (Peprotech), 20 ng/mL mouse EGF (Peprotech)]. After 7 days, the spheroid efficiency was determined.
Yang M., Arai A., Udagawa N., Hiraga T., Lijuan Z., Ito S., Komori T., Moriishi T., Matsuo K., Shimoda K., Zahalka A.H., Kobayashi Y., Takahashi N, & Mizoguchi T. (2017). Osteogenic Factor Runx2 Marks a Subset of Leptin Receptor-Positive Cells that Sit Atop the Bone Marrow Stromal Cell Hierarchy. Scientific Reports, 7, 4928.
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2

PDGF-BB Stimulation of VSMCs

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VSMCs were serum starved (0% FCS) for 16 hours, and then stimulated with 10 ng/ml mouse PDGF (platelet derived growth factor)-BB (Peprotech) for 48 hours. Edu (10 mg/mL) was added 4 hours before harvesting. Cells were then fixed and stained with ApollAlexa 488 according to the manufacturer’s instructions (RiboBio). The percentages of EdU-positive VSMCS were determined by flow cytometry.
Peng Y., Wang J., Zhang M., Niu P., Yang M., Yang Y, & Zhao Y. (2016). Inactivation of Semicarbazide-Sensitive Amine Oxidase Stabilizes the Established Atherosclerotic Lesions via Inducing the Phenotypic Switch of Smooth Muscle Cells. PLoS ONE, 11(4), e0152758.
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