Quattro premier xe system

The morphologies of UCPP and UCNPs were examined by transmission electronic microscopy (TEM, Tecnai G2spirit Biotwin) at an accelerating voltage of 120 kV. The platinum content obtained outside of the dialysis bags in drug release experiments was measured on Inductively Coupled Plasma Mass Spectrometer (ICP-MS, iCAP Qc-ICPMS, Thermoscientific, USA) and Inductively Coupled Plasma Optical Emission Spectrometer (ICP-OES, iCAP 6300, Thermoscientific, USA). ¹H NMR spectra were measured by a Unity-300 MHz NMR spectrometer (Bruker) at room temperature. UV-vis absorption spectra were taken on a Milton Ray Spectronic 3000 array spectrophotometer. Photoluminescence (PL) spectra were measured on a Perkin-Elmer LS-55 spectro-fluorometer. Mass Spectroscopy (ESI-MS) measurements were performed on a Quattro Premier XE system (Waters) equipped with an electrospray interface (ESI). Size and size distribution of micelles were determined by DLS (Zetasizer nano ZS, Malvern, UK). Fourier transform infrared (FTIR) spectra were recorded on a Paragon 1,000 instrument by KBr sample holder method. Oxygen concentration was measured by a oxygen dissolving meter (HI-2400, HANNA, Italy).

Recombinant hPH20 was purified using a Ni-NTA column (GE Healthcare, Shanghai, China). An aliquot of 100 μl of recombinant hPH20 was mixed with 80 μl of purified o-HAs (HA₄^NA, HA₆^NA, and HA₈^NA), respectively, and incubated at 37°C for 8 h; then recombinant hPH20 was inactivated by boiling for 5 min. To remove the salt, 180 μl of methanol was added to the reaction system, mixed gently, and then centrifuged at 12,000 rpm for 10 min. Electrospray ionization-mass spectrometry (ESI-MS) spectra were acquired using a Quattro Premier XE system (Waters). Additionally, HPLC separation was performed using a Zorbax NH₂ column (4.6 × 250 mm) at 40°C. The products were eluted with acetonitrile/water (75:25) at a flow rate of 1.0 ml min⁻¹. The eluent was monitored by measuring the total ions in the mass range of m/z 100–600 in the negative mode.

¹H-NMR spectra were measured by a Unity-300 MHz NMR spectrometer (Bruker) at room temperature. Fourier Transform infrared (FT-IR) spectroscopy was performed using a Bruker Vertex 70 spectrometer. A Quattro Premier XE system (Waters) equipped with an electrospray interface was used for the mass spectroscopy (ESI-MS) assessments. The total platinum (Pt) content in the polymer-Pt(IV) conjugates and in samples obtained from the dialysis bags in drug release experiments was determined by inductively coupled plasma optical emission spectrometer (ICP-OES, iCAP 6300, ThermoScientific, United States). The quantitative determination of trace levels of Pt was measured by ICP-MS (Xseries II, ThermoScientific, United States). Size and size distribution of micelles were determined by dynamic light scattering (DLS) with a vertically polarized He–Ne laser (DAWN EOS, Wyatt Technology, United States). The morphology of the polymer micelles was evaluated by transmission electron microscope (TEM) performed on a JEOL JEM-1011 electron microscope. Particle size and zeta potential measurements were conducted on a Malvern Zetasizer Nano ZS. (Zetasizer Nano ZS is a high performance dual angle particle size and molecular size analyzer that uses DLS combined with “NIBS” optics to enhance the detection of aggregates, as well as small or diluted samples, and poles. Low or high concentration sample).