Favorprep total rna mini kit

Five tissue sections were cut from FFPE blocks and transferred to one microcentrifuge tube per isolation. Deparaffinization was performed with 1 mL xylene for 10 min, twice, followed by washing with 1 mL absolute ethanol for 10 min, twice. Total RNAs were isolated from three air-dried deparaffinized sections per isolation using the FavorPrep Total RNA Mini Kit (cat no. FABRK001, Favorgen Biotech, Pingtung, Taiwan) according to the manufacturer’s protocol. The purity and the concentration of RNA in the samples were measured by nanodrop (Thermo Fisher Scientific, Waltham, MA, USA). Up to the analysis, the RNA samples were stored at −80 °C.

The RNA was extracted from fresh tissues using the FavorPrep Total RNA Mini Kit (cat no. FABRK001, Favorgen Biotech, Pingtung, Taiwan) according to the manufacturer’s protocol. Briefly, after homogenizing the samples in 1 mL YTzol pure RNA (cat no. YT9064, Yekta Tajhiz Azma, Tehran, Iran), 200 μL of chloroform was added to the lysate, shaken vigorously for 30 s, and after 3 min of incubation at room temperature, centrifuged at 12,000× g for 10 min at 4 °C. The supernatant was aspirated and 500 µL ethanol (100%) was added. Then, RNA washing was performed using 750 µL of washing buffer (ethanol 80%). Forty microliters of RNase-free ddH2O were added and the samples were centrifuged at 18,000× g for 1 min to elute RNA. The purity and concentration of RNA in the samples was measured by nanodrop (Thermo Fisher Scientific, Waltham, MA, USA). Until the final analysis, the RNA samples were stored at −80 °C.