Cobas integra reagents

Baseline demographic and clinical data were retrieved electronically from the medical records of the general hospital registry and reviewed retrospectively. For MM patients with multiple admissions, only the first set of observation data was used, thus preserving the assumption of independence of observations.
The following indicators were evaluated: 1) demographic characteristics, including age, sex, and underlying disease (hypertension and diabetes); 2) laboratory data, including hemoglobin, serum calcium, serum creatinine, albumin, globulin, serum/urine light chain protein, lactate dehydrogenase (LDH), and beta-2 microglobulin; 3) anti-myeloma therapy regimen; 4) duration of follow-up; 5) serum creatinine at the last follow-up evaluation, time of death, or dialysis; 6) serum calcium and albumin levels (Cobas Integra reagents; Roche Diagnostics, Switzerland).
Albumin-adjusted serum calcium was calculated using the following formula: serum albumin-corrected calcium (mg/dL) = total calcium (mg/dL) + 0.8 × [4 - albumin (g/dL)] [18 (link)–20 (link)].

The blood concentration of HbA_1C was determined using a turbidimetric immunoinhibition method. The concentrations of triglycerides (TG), total cholesterol, high-density and low-density lipoprotein cholesterol fractions (HDL, LDL) were assessed by standard enzymatic-colorimetric methods. The laboratory tests were conducted at baseline and at week 12 using the Roche device and Cobas Integra Reagents (Roche Diagnostics, Warszawa, Poland). Participants’ blood pressure and the cardiac hemodynamic parameters (the left ventricular ejection fraction (LVEF) and the left ventricular end-systolic and end-diastolic dimensions (LVEDD, LVESD)) were measured by one- and two-dimensional echocardiography with the Vivid 7 ultrasound scanner (General Electric, Waukesha, WI, USA). Average daily systolic and diastolic blood pressures (BPs, BPd) were calculated after 24-h ambulatory blood pressure monitoring (24-h ABPM) with the Boso Tm 2430 device (Bosch and Sohn, Jungingen, Germany).