OptiPrep, Histopaque 1077, fMLP, IL8, and LY294002 were obtained from Sigma-Aldrich (St. Louis, Missouri). LTB4, MK886, and LY223982 were purchased from Cayman Chemical (Ann Arbor, Michigan). Anti-5-LO, anti-p-Akt (clone C31E5E for S473), anti-AKT, anti-MLCII, anti-pMLCII (Ser19), anti-total ERK1/2, and anti-p-Erk1/2 (clone D13.14.4E) rabbit antibodies were all purchased from Cell Signaling Technology (Beverly, Massachusetts). Anti-MMP9, anti-myeloperoxidase, anti-CD63, anti-LAMP1, and anti-GM130 were obtained from Abcam (Cambridge, Massachusetts). CD11b APC antibody was purchase from BD Biosciences (San Jose, California).
Ly223982
Manufactured by Cayman Chemical
LY223982 is a chemical compound used in laboratory settings. It serves as a tool for researchers and scientists to facilitate their experiments and analyses. The core function of LY223982 is to provide a specific chemical structure or property that can be utilized in various research applications.
Automatically generated - may contain errors
Lab products found in correlation
Anti lamp1, by Abcam (2 mentions)
Optiprep, by Merck Group (2 mentions)
Anti p erk1 2 clone d13.14.4e, by Cell Signaling Technology (2 mentions)
Anti total erk1 2, by Cell Signaling Technology (2 mentions)
Mk886, by Cayman Chemical (2 mentions)
Anti 5 lo, by Cell Signaling Technology (2 mentions)
Cd11b apc antibody, by BD (2 mentions)
Anti cd63, by Abcam (2 mentions)
Anti gm130, by Abcam (2 mentions)
Formyl methionyl leucyl phenylalanine (fmlp), by Merck Group (2 mentions)
Histopaque 1077, by Merck Group (2 mentions)
Ly294002, by Merck Group (2 mentions)
Anti mmp9, by Abcam (2 mentions)
Anti myeloperoxidase, by Abcam (1 mentions)
Anti akt, by Cell Signaling Technology (1 mentions)
Rhodamine phalloidin, by Thermo Fisher Scientific (1 mentions)
Celltracker red cmtpx, by Thermo Fisher Scientific (1 mentions)
Dynasore, by Bio-Techne (1 mentions)
Mitmab, by Bio-Techne (1 mentions)
Celltracker green cmfda, by Thermo Fisher Scientific (1 mentions)
3 protocols using ly223982
1
Neutrophil Activation and Signaling Pathways
2
Multicolor Fluorescent Immune Cell Imaging
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Antibodies against mouse Itgb2 (clone M18/2; Alexa Fluor 594 conjugated), NMIIA heavy chain (clone Poly19098), and custom rhodamine-conjugated antibody to CD31 (clone 390) were obtained from BioLegend, Inc. Antibodies against CD31 (clone 390; eFluor 450 conjugated) and for quenching Alexa Fluor 488 (RRID AB_2532697) were purchased from Invitrogen. Antibodies against CD16/32 (Fc block; clone 2.4G2) and ITGB2 (clone CTB104; Alexa Fluor 488 conjugated) were obtained from BD PharMingen and Santa Cruz Biotechnology Inc., respectively. E. coli BPs (K12-strain; Texas red conjugated), CellTracker Green CMFDA, CellTracker Red CMTPX, CellMask Deep Red for PM, and rhodamine phalloidin dyes were obtained from Molecular Probes. PitStop2, monodansylcadaverine (MDC), fibrinogen (human plasma derived), and WKYMVm were purchased from MilliporeSigma. Recombinant fNLFNYK, human C5a, and human CXCL8 were obtained from Santa Cruz Biotechnology Inc., R&D Systems, and PeproTech Inc., respectively. LTB4, LY223982, Y27632, nBleb, and GW4869 were purchased from Cayman Chemical. nBleb used in this study is a photostable version of blebbistatin (Lucas-Lopez et al., 2005 ). MK886, Nexinhib20, Exo1, dynasore, and MitMAB were obtained from Tocris Bioscience.
3
Neutrophil Activation and Signaling Pathways
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OptiPrep, Histopaque 1077, fMLP, IL8, and LY294002 were obtained from Sigma-Aldrich (St. Louis, MO). LTB4, MK886 and LY223982 were purchased from Cayman Chemical (Ann Arbor, MI). Anti-5-LO, Anti-p-Akt (clone C31E5E for S473), anti-AKT, anti-myosin light chain 2, anti-p-myosin light chain 2 (Ser19), anti-total ERK1/2, and anti-p-Erk1/2 (clone D13.14.4E) rabbit antibodies were all purchased from Cell Signaling Technology (Beverly, MA). Anti-MMP9, anti-MPO, anti-CD63, anti-LAMP1, and anti-GM130 were obtained from Abcam (Cambridge. MA). CD11b APC antibody was purchase from BD Biosciences (San Jose, CA).
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