Single stranded 2 o methyl enhanced mir 92a or control inhibitor

Manufactured by GenePharma

Sourced in China

The Single-stranded 2′ O-methyl enhanced miR-92a or control inhibitor is a laboratory reagent used to inhibit the function of the miR-92a microRNA in experimental settings. It is a synthetic, chemically modified oligonucleotide designed to bind and neutralize the target microRNA.

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Lab products found in correlation

Neon electroporation transfection system, by Thermo Fisher Scientific (2 mentions)

Spelling variants of this product

Inhibitor control (70 citations) MiR-control (37 citations) MiR‐92a inhibitor (4 citations) MiR inhibitor control (4 citations)

2 protocols using single stranded 2 o methyl enhanced mir 92a or control inhibitor

Overexpression and Inhibition of miR-92a in HUVECs

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Pre-miR microRNA are double-stranded RNA mimics, which can be introduced into target cells using electroporation to mimic endogenous mature microRNA molecular in cells. For miRNA overexpression experiments, 100nM pre-miR-92a mimic (GenePharma, China) or pre-miR-scramble control (NC), was transfected into HUVEC in 100 μL “R buffer” using Neon electroporation transfection system (Invitrogen) operating at 1350 V with one 30 ms pulse. For miRNA anti-sense inhibitor experiments, 100nM single-stranded 2′ O-methyl enhanced miR-92a or control inhibitor (GenePharma, China) was transfected into HUVEC using Neon transfection system.

Zhang L., Zhou M., Qin G., Weintraub N.L, & Tang Y. (2014). MiR-92a regulates viability and angiogenesis of endothelial cells under oxidative stress. Biochemical and biophysical research communications, 446(4), 952-958.

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miRNA overexpression and inhibition in VSMC

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For miRNA overexpression experiments, 100 nM double-stranded miR-92a mimic (GenePharma, China) or control miRNA (control) in 100 µL “R buffer” was transfected into VSMC using the Neon electroporation transfection system (Invitrogen). Settings were optimized at 1,400 V with two 20 ms pulses. For miRNA anti-sense inhibitor experiments, 100 nM single-stranded 2′O-methyl enhanced miR-92a or control inhibitor (GenePharma, China) was transfected into VSMC using Neon transfection system.

Zhang L., Zhou M., Wang Y., Huang W., Qin G., Weintraub N.L, & Tang Y. (2014). miR-92a inhibits vascular smooth muscle cell apoptosis: role of the MKK4–JNK pathway. Apoptosis : an international journal on programmed cell death, 19(6), 975-983.

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