Erk1 2 phospho thr202 tyr204

Manufactured by BioLegend

ERK1/2 Phospho (Thr202/Tyr204) is a lab equipment product that detects the phosphorylation of ERK1/2 at the Thr202 and Tyr204 residues. It is used to monitor the activation of the ERK1/2 signaling pathway.

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Lab products found in correlation

True nuclear transcription factor buffer set, by BioLegend (1 mentions) F4 80 bm8, by BioLegend (1 mentions) Galectin 3, by BioLegend (1 mentions) Stat3 phospho, by BioLegend (1 mentions) Facsaria 3 instrument, by BD (1 mentions) Cd11b, by BioLegend (1 mentions)

1 protocol using erk1 2 phospho thr202 tyr204

Multiparametric Flow Cytometry Analysis

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We performed flow cytometric analysis using a FACSAria III instrument (BD Biosciences) and analyzed the data using FlowJo software (Tree Star Inc, Ashland, OR). We used antibodies that had specificity to the following proteins: F4/80 (BM8; BioLegend), Ly6G (1A‐8‐Ly6g; BioLegend), CD11b (M1/70; BioLegend), galectin‐3 (M3/38; BioLegend), MERTK (Mer; 2B10C42; BioLegend), CD206 (15‐2; BioLegend), ERK1/2 Phospho (Thr202/Tyr204) (6B8B69; BioLegend) and STAT3 Phospho (Tyr705) (13A3‐1; BioLegend). We analyzed galectin‐3 (M3/38; BioLegend) using a True‐Nuclear Transcription Factor Buffer Set (BioLegend), ERK1/2 Phospho (Thr202/Tyr204) (6B8B69; BioLegend) and STAT3 Phospho (Tyr705) (13A3‐1; BioLegend) using ice‐cold 90% methanol after fixation.

Shirakawa K., Endo J., Kataoka M., Katsumata Y., Anzai A., Moriyama H., Kitakata H., Hiraide T., Ko S., Goto S., Ichihara G., Fukuda K., Minamino T, & Sano M. (2020). MerTK Expression and ERK Activation Are Essential for the Functional Maturation of Osteopontin‐Producing Reparative Macrophages After Myocardial Infarction. Journal of the American Heart Association: Cardiovascular and Cerebrovascular Disease, 9(18), e017071.

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