Annexin 5 fitc and propidium iodide staining solution

Manufactured by Thermo Fisher Scientific

Annexin V-FITC and propidium iodide staining solution is a laboratory reagent used for the detection and analysis of apoptosis and cell death in various cell types. Annexin V binds to phosphatidylserine, which is exposed on the cell surface during apoptosis, while propidium iodide stains the DNA of cells with compromised cell membranes, indicating late-stage apoptosis or necrosis. This solution enables the differentiation of viable, early apoptotic, and late apoptotic/necrotic cells through flow cytometry or fluorescence microscopy.

Automatically generated - may contain errors

Lab products found in correlation

Facscalibur flow cytometer, by BD (1 mentions) Cd4 cd25 regulatory t cell isolation kit, by Miltenyi Biotec (1 mentions) Annexin 5 fitc binding solution, by Thermo Fisher Scientific (1 mentions) Facscalibur, by BD (1 mentions)

Close spelling variants of this product

FITC-labeled Annexin V and Propidium Iodide Staining Solution Annexin V-FITC/propidium iodide (PI) staining Annexin V-FITC and propidium iodide Annexin V/propidium iodide staining Annexin V FITC/propidium iodide (PI) Annexin V and propidium iodide Propidium Iodide Staining Solution Annexin V/propidium iodide Annexin V-FITC staining Propidium iodide staining

2 protocols using annexin 5 fitc and propidium iodide staining solution

Regulatory T Cell Quantification

Check if the same lab product or an alternative is used in the 5 most similar protocols

The number of Treg cells in each collected sample was counted by using an Annexin V-FITC and propidium iodide staining solution (Thermo Fisher Scientific, Waltham, MA) in reference with the recommended assay protocol provided on the manufacturer’s manual. The Treg cells were isolated using a CD4+CD25+ Regulatory T Cell Isolation Kit (Miltenyi Biotec, Germany) following instruction provided by the manufacturer. The result reading was carried out in a FACS Calibur flow cytometer (BD, San Jose, CA).

Huang Z., Liu F., Wang W., Ouyang S., Sang T., Huang Z., Liao L, & Wu J. (2021). Deregulation of circ_003912 contributes to pathogenesis of erosive oral lichen planus by via sponging microRNA-123, -647 and -31 and upregulating FOXP3. Molecular Medicine, 27, 132.

+ Open protocol

+ Expand

Assessing Apoptosis in A549 Cells

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

The A549 cells in the logarithmic growth phase were treated with 125, 250, and 500 μg/mL LLTPs for 48 h. Then, the cells were washed with phosphate-buffered saline (PBS) three times, suspended in annexin V–FITC binding solution, mixed with annexin V–FITC and propidium iodide staining solution (Thermo Fisher Scientific, Inc.), and cultured at 4°C in the dark for 15 min. Finally, the apoptosis of cancer cells was analyzed by flow cytometry (FACSCalibur, BD, NJ, USA) [15 (link)].

Chen J., Du Y., Long Y., Tao D., Hu M., Jiang Y., Wan Y, & Yang D. (2022). Polyphenols in Ilex latifolia Thunb. inhibit human lung cancer cell line A549 by regulation of the PI3K-Akt signaling pathway. BMC Complementary Medicine and Therapies, 22, 85.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!