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Goat anti hamster igg h l alexa fluor 488

Manufactured by Thermo Fisher Scientific
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Goat anti-hamster IgG (H + L) Alexa Fluor 488 is a secondary antibody conjugated with Alexa Fluor 488 dye. It is designed to detect and bind to hamster immunoglobulin G (IgG) heavy and light chains.

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Lab products found in correlation

Lsrii analytic cytometer, by BD (2 mentions)

Spelling variants of this product

Alexa Fluor 488 anti-goat IgG (28 citations) Alexa Fluor 488 goat anti (9 citations) Goat anti-hamster IgG (6 citations) Alexa Fluor 488 anti (6 citations) Goat anti-hamster IgG (H+L, (3 citations) Alexa-488 anti-goat (3 citations) Alexa Fluor IgG H + L (2 citations) Alexa Fluor 488 anti- IgG (2 citations) Anti-hamster Alexa 488 (2 citations) Alexa Fluor H + L (2 citations)

2 protocols using goat anti hamster igg h l alexa fluor 488

1

SARS-CoV-2 Spike Protein Expression in Expi293F Cells

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SARS-CoV-2 S-expressing expi293F cells were generated by transfection with linearized plasmid (pcDNA3.1) encoding codon-optimized full-length SARS-CoV-2 S protein matching the amino acid sequence of the IL-CDC-IL1/2020 isolate (GenBank ACC# MN988713), the B.1.1.7 isolate9 , or the B.1.351 isolate37 (link). Stable transfectants were single-cell sorted and selected to obtain a high-level Spike surface expressing clone (293F-Spike-S2A). 293F-Spike-S2A cells were incubated with 100 μl of plasma diluted 100-fold in RPMI containing 10% FBS (R10) for 30 min at 37 °C. Cells were washed three times and stained with a goat anti-hamster IgG (H + L) Alexa Fluor 488 diluted 1:250 (Cat A-21110, ThermoFisher Scientific). Cells were then fixed with 4% formaldehyde solution and fluorescence was evaluated on a LSRII analytic cytometer (BD Bioscience).
Wuertz K.M., Barkei E.K., Chen W.H., Martinez E.J., Lakhal-Naouar I., Jagodzinski L.L., Paquin-Proulx D., Gromowski G.D., Swafford I., Ganesh A., Dong M., Zeng X., Thomas P.V., Sankhala R.S., Hajduczki A., Peterson C.E., Kuklis C., Soman S., Wieczorek L., Zemil M., Anderson A., Darden J., Hernandez H., Grove H., Dussupt V., Hack H., de la Barrera R., Zarling S., Wood J.F., Froude J.W., Gagne M., Henry A.R., Mokhtari E.B., Mudvari P., Krebs S.J., Pekosz A.S., Currier J.R., Kar S., Porto M., Winn A., Radzyminski K., Lewis M.G., Vasan S., Suthar M., Polonis V.R., Matyas G.R., Boritz E.A., Douek D.C., Seder R.A., Daye S.P., Rao M., Peel S.A., Joyce M.G., Bolton D.L., Michael N.L, & Modjarrad K. (2021). A SARS-CoV-2 spike ferritin nanoparticle vaccine protects hamsters against Alpha and Beta virus variant challenge. NPJ Vaccines, 6, 129.
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2

SARS-CoV-2 Spike Protein Expression and Antibody Binding Assay

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SARS-CoV-2 S-expressing expi293F cells were generated by transfection with linearized plasmid (pcDNA3.1) encoding codon-optimized full-length SARS-CoV-2 S protein matching the amino acid sequence of the IL-CDC-IL1/2020 isolate (GenBank ACC# MN988713), the B.1.1.7 isolate9 , or the B.1.351 isolate41 (link). Stable transfectants were single-cell sorted and selected to obtain a high-level Spike surface expressing clone (293F-Spike-S2A). 293F-Spike-S2A cells were incubated with 100 μl of plasma diluted 100-fold in RPMI containing 10% FBS (R10) for 30 minutes at 37°C. Cells were washed 3 times and stained with a goat anti-hamster IgG (H+L) Alexa Fluor 488 (ThermoFisher Scientific). Cells were then fixed with 4% formaldehyde solution and fluorescence was evaluated on a LSRII analytic cytometer (BD Bioscience).
Wuertz K.M., Barkei E.K., Chen W.H., Martinez E.J., Lakhal-Naouar I., Jagodzinski L.L., Paquin-Proulx D., Gromowski G.D., Swafford I., Ganesh A., Dong M., Zeng X., Thomas P.V., Sankhala R.S., Hajduczki A., Peterson C.E., Kuklis C., Soman S., Wieczorek L., Zemil M., Anderson A., Darden J., Hernandez H., Grove H., Dussupt V., Hack H., de la Barrera R., Zarling S., Wood J.F., Froude J.W., Gagne M., Henry A.R., Mokhtari E.B., Mudvari P., Krebs S.J., Pekosz A.S., Currier J.R., Kar S., Porto M., Winn A., Radzyminski K., Lewis M.G., Vasan S., Suthar M., Polonis V.R., Matyas G.R., Boritz E.A., Douek D.C., Seder R.A., Daye S.P., Rao M., Peel S.A., Joyce M.G., Bolton D.L., Michael N.L, & Modjarrad K. (2021). A SARS-CoV-2 spike ferritin nanoparticle vaccine protects against heterologous challenge with B.1.1.7 and B.1.351 virus variants in Syrian golden hamsters. bioRxiv.
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