Elaidate

Manufactured by Merck Group

Sourced in United States

Elaidate is a laboratory equipment designed for the analysis and measurement of various samples. It functions as a reliable tool for researchers and scientists in a variety of fields, providing accurate and consistent results.

Automatically generated - may contain errors

Lab products found in correlation

6 protocols using elaidate

Lipid Standards for Cell Culture

Check if the same lab product or an alternative is used in the 5 most similar protocols

Culture medium and supplements were purchased from Thermo Fisher Scientific (Waltham, MA, USA). Oleate, palmitate, stearate, linOleate, elaidate, vaccenate, bovine serum albumin, HepG2, HEK293T, and SK-N-FI cells were purchased from Sigma-Aldrich (St. Louis, MO, USA). All of the chemicals that were used in this study were of analytical grade. All of the experiments and measurements were carried out by using Millipore ultrapure water.

Zámbó V., Orosz G., Szabó L., Tibori K., Sipeki S., Molnár K., Csala M, & Kereszturi É. (2022). A Single Nucleotide Polymorphism (rs3811792) Affecting Human SCD5 Promoter Activity Is Associated with Diabetes Mellitus. Genes, 13(10), 1784.

+ Open protocol

+ Expand

Lipid Standards for Metabolic Analyses

Check if the same lab product or an alternative is used in the 5 most similar protocols

The culture medium and supplements were purchased from Thermo Fisher Scientific (Waltham, MA, USA). Palmitate, oleate, elaidate, vaccenate, FA free bovine serum albumin (BSA), trans-vaccenic acid methyl ester, methyl oleate methyl Palmitate, methyl palmitoleate methyl stearate, 1,2-dipalmitoyl-rac-glycerol (>99%), 1-palmitoyl-2-oleoyl-sn-glycerol (>99%), 1,2-dioleoyl-sn-glycerol (>97%) and 1-octadecanoyl-2-hexandecanoyl-sn-glycerol (>99%) were from Sigma Aldrich (St. Louis, MO, USA), n-hexane was purchased from Romil (Waterbeach, UK). C16:0, C17:0, C18:0, C18:1(9Z) (>99%) ceramides were purchased from Avanti Polar Lipids Inc. (Alabaster, AL, USA).Methanol (gradient grade) and acetonitrile (gradient grade) were purchased from Merck KGaA. (Darmstadt, Germany). All other chemicals used in this study were of analytical grade. All experiments and measurements were carried out by using Milipore (Darmstadt, Germany) ultrapure water.

Sarnyai F., Somogyi A., Gór-Nagy Z., Zámbó V., Szelényi P., Mátyási J., Simon-Szabó L., Kereszturi É., Tóth B, & Csala M. (2020). Effect of cis- and trans-Monounsaturated Fatty Acids on Palmitate Toxicity and on Palmitate-induced Accumulation of Ceramides and Diglycerides. International Journal of Molecular Sciences, 21(7), 2626.

+ Open protocol

+ Expand

Fatty Acid Uptake Assay in Cell Lines

Check if the same lab product or an alternative is used in the 5 most similar protocols

Culture medium and supplements were purchased from Thermo Fisher Scientific (Waltham, MA, USA). Oleate, palmitate, stearate, linOleate, elaidate, vaccenate, bovine serum albumin, HEK293T and HepG2 cells were purchased from Sigma-Aldrich (St. Louis, MO, USA). All chemicals used in the study were of analytical grade. All experiments and measurements were performed using Millipore ultrapure water.

Tibori K., Zámbó V., Orosz G., Szelényi P., Sarnyai F., Tamási V., Rónai Z., Csala M, & Kereszturi É. (2024). Allele-specific effect of various dietary fatty acids and ETS1 transcription factor on SCD1 expression. Scientific Reports, 14, 177.

+ Open protocol

+ Expand

Fatty Acid Treatment Optimization

Check if the same lab product or an alternative is used in the 5 most similar protocols

Palmitate, elaidate, oleate and vaccenate (Sigma, Burlington, MA, USA) were diluted in ethanol (Molar Chemicals, Budapest, Hungary) to a concentration of 100 mM, conjugated with 20% FA free BSA (Sigma, Burlington, MA, USA) in a 1:4 ratio at 50 °C for 1 h. The working solution for FA treatments was always freshly prepared in FBS-free and antibiotic-free medium in a 0.2, 0.4 or 0.8 mM final concentration. The culture medium was replaced by FBS-free and antibiotic-free medium at 1 h before the cells were treated with FAs for 8–24 h at 70–80% confluence in 6-well plates (for Western blot; qPCR; electron microscopy and analysis of ceramides, DGs, TGs and fatty acid profile) or in 96-well plates (for cell viability assay and the detection of apoptosis and necrosis).

Sarnyai F., Kereszturi É., Szirmai K., Mátyási J., Al-Hag J.I., Csizmadia T., Lőw P., Szelényi P., Tamási V., Tibori K., Zámbó V., Tóth B, & Csala M. (2022). Different Metabolism and Toxicity of TRANS Fatty Acids, Elaidate and Vaccenate Compared to Cis-Oleate in HepG2 Cells. International Journal of Molecular Sciences, 23(13), 7298.

+ Open protocol

+ Expand

Lipid Metabolic Profiling Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols

Culture medium and supplements were purchased from Thermo Scientific (USA). Palmitate, oleate, elaidate, vaccenate, fatty acid free bovine serum albumin, trans-vaccenic acid methyl ester, methyl oleate methyl Palmitate, methyl palmitoleate methyl stearate, 1,2-dipalmitoyl-racglycerol (>99%), 1-palmitoyl-2-oleoyl-sn-glycerol (>99%), 1,2-dioleoyl-sn-glycerol (>97%), 1-octadecanoyl-2-hexandecanoyl-sn-glycerol (>99%), NADH (≥97%), and sodium pyruvate (>99%) were from Sigma Aldrich (St. Louis, MO), n-hexane was purchased from Romil (UK). C16:0, C17:0, C18:0, C18:1(9Z) (>99%) ceramides were purchased from Avanti Polar Lipids Inc (Alabaster, AL). Methanol (gradient grade) and acetonitrile (gradient grade) were purchased from Merck (Germany). All other chemicals used in this study were of analytical grade. All experiments and measurements were carried out by using Millipore ultrapure water.

Sarnyai F., Donkó M.B., Mátyási J., Gór-Nagy Z., Marczi I., Simon-Szabó L., Zámbó V., Somogyi A., Csizmadia T., Lőw P., Szelényi P., Kereszturi É., Tóth B, & Csala M. (2019). Cellular toxicity of dietary trans fatty acids and its correlation with ceramide and diglyceride accumulation. Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association, 124.

+ Open protocol

+ Expand

Fatty Acid Profiling and Cell Assays

Check if the same lab product or an alternative is used in the 5 most similar protocols

Palmitate, elaidate, oleate and vaccenate (Sigma) were diluted in isopropanol (Molar Chemicals) to a concentration of 50 mM, conjugated with 4.16 mM fatty acid free BSA (Sigma) in 1:4 ratio, on 37 °C for 1 hour. The working solution for fatty acid treatments was always prepared freshly in FBS-free and antibiotic-free medium at 0.25 or 0.5 mM final concentration.
The culture medium had been replaced by FBS-free and antibiotic-free medium for 1 hour before the cells were treated with fatty acids for 4-24 hour at 70-80% confluence in 6-well plates (for western blot, RT-PCR and analysis of ceramides, DGs and fatty acid profile) or in 96-well plates (for cell viability assay and detection of apoptosis and necrosis).

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!