To evaluate the impact of shipping condition on CDC, viability and cell count of effector cells, we analyzed both serum and whole blood samples of a healthy donor (D1) stored under different conditions or subjected to five freeze-thaw cycles. The serum samples were prepared as described above. For evaluation of repeated freezing and thawing or storing at RT (up to 96 h), serum samples aliquots containing two defined ch14.18/CHO concentrations (1 and 0.1 µg/ml) were used. For evaluation of leukocyte viability and cell count, whole blood samples were collected in either BD Vacutainer plastic K2EDTA- (BD Biosciences, Heidelberg, Germany) or BD Vacutainer plastic sodium-heparin-containing tubes (BD Biosciences, Heidelberg, Germany) and stored at RT for up to 72 h. CDC was evaluated using the established cytotoxicity assay. Rituximab containing samples and Ab-free sera were used as negative controls. Viability of leukocytes was evaluated using trypan blue test and a cell count with automated cell counter (EMD Millipore Corporation, Billerica, MA, USA).
Vacutainer plastic k2edta
Manufactured by BD
Sourced in Germany, United States
The BD Vacutainer plastic K2EDTA- is a blood collection tube used for collecting and storing whole blood samples. It contains the anticoagulant K2EDTA, which prevents the blood from clotting during the collection and storage process.
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Lab products found in correlation
2 protocols using vacutainer plastic k2edta
1
Evaluating Impact of Shipping Conditions on Effector Cells
2
Analytical and Clinical Evaluation of Calprotectin Assay
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An evaluation protocol using quality control materials provided by the manufacturer and blood samples (serum/plasma) was designed. Blood samples were obtained by venipuncture using serum separator tubes (SST) (BD Vacutainer® SST™ II Advance) and spray‐coated K2 Ethylenediaminetetraacetic acid tubes (EDTA) (BD Vacutainer™ Plastic K2EDTA) (BD, USA). Both types of samples were centrifuged 8 minutes at 2000 g and frozen at −20ºC until the analysis.
The protocol included an analytical evaluation phase in which imprecision, linearity of dilution, differences between serum and plasma samples and method comparison were examined. A clinical evaluation was also performed, in which serum calprotectin concentrations in active and remission RA patients were compared. According to DiaSorin Liaison® Calprotectin assay range and the expected serum calprotectin concentrations, patients’ samples were analyzed using 1:5 as initial dilution factor.
The protocol included an analytical evaluation phase in which imprecision, linearity of dilution, differences between serum and plasma samples and method comparison were examined. A clinical evaluation was also performed, in which serum calprotectin concentrations in active and remission RA patients were compared. According to DiaSorin Liaison® Calprotectin assay range and the expected serum calprotectin concentrations, patients’ samples were analyzed using 1:5 as initial dilution factor.
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