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Pe conjugated mouse anti human cd144

Manufactured by BD

The PE-conjugated mouse anti-human CD144 is a laboratory reagent used for the detection and identification of CD144 (also known as VE-cadherin) expressed on human cells. It is a mouse monoclonal antibody conjugated to the fluorescent dye phycoerythrin (PE).

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2 protocols using pe conjugated mouse anti human cd144

1

Stem Cell Derivation and Characterization

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Endothelial cells and mesenchymal cells were derived as described previously15 (link). iPSC-NSC were derived as described previously31 (link). Gene expression was evaluated using qPCR and flow cytometry. Following antibodies were used for flow cytometric analysis: PE-conjugated mouse anti-human CD140b (PDGFRB; 558821, BD Biosciences); APC-conjugated anti-human CD166 (ALCAM; 130-119-809, Miltenyi Biotec); anti- human CD105, human CD73, and human CD90 (MSC phenotyping kit; 130-095-198, Miltenyi Biotec); FITC-conjugated mouse anti-human CD31 (557508, BD Biosciences); PE-conjugated mouse anti-human CD144 (561714, BD Biosciences).
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2

Derivation and Characterization of iPSC-NCCs

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ECs and MCs were derived as described previously13 (link). iPSC-NCCs were derived using ectoderm-derived lineages, as described previously25 (link),26 (link). B27 was replaced with 20% AS400 to supplement the CD-AOF medium. Following antibodies were used for flow cytometric analysis: PE-conjugated mouse anti-human CD140b (PDGFRB; 558821, BD Biosciences); APC-conjugated anti-human CD166 (ALCAM; 130-119-809, Miltenyi Biotec); anti- human CD105, human CD73, and human CD90 (MSC phenotyping kit; 130-095-198, Miltenyi Biotec); FITC-conjugated mouse anti-human CD31 (557508, BD Biosciences); PE-conjugated mouse anti-human CD144 (561714, BD Biosciences).
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