Rabbit anti aldh1a1

For histochemistry or immunohistochemistry, the tissues were fixed in 4% paraformaldehyde (PFA), dehydrated in a series of graded ethanol solutions, embedded in paraffin, and cut into 5 μm sections using a rotary microtome (Leica, Germany). The sections were then used for subsequent H&E staining or immunofluorescence microscopy. The antibodies used for immunohistochemistry were rabbit anti-CD133 (1:100 dilution, Proteintech), rabbit anti-ALDH1A1(1:100 dilution, Proteintech), rabbit anti-ABCG2 (1:100 dilution, Cell Signaling Technology), rabbit anti-β-catenin (1:100 dilution, Proteintech), rabbit anti-SOD2 (1:100 dilution, Proteintech) and rabbit anti-GPX4 (1:100 dilution, Abcam). Images were analyzed using the ImageJ program.

The cells were collected in a lysis buffer [24 (link)]. The protein expression was measured using semi-quantitative western blotting. Rabbit anti-pSmad2 (Ser465/467) (Merck, Darmstadt, Germany), rabbit anti-Smad2, rabbit anti-pTR2 (Ser225) (Abcam, Cambridge, UK), rabbit anti-TGFBR1 (TR1), rabbit anti-TGFBR2 (Santa Cruz Biotechnology, Dallas, TX, USA), rabbit anti-pTR1 (Ser165) (Aviva Systems Biology, San Diego, CA, USA), rabbit anti-Slug (Cell Signaling), rabbit anti-N-cadherin (GeneTex, Inc., Irvine, CA, USA), rabbit anti-ALDH1A1 (protein tech), rabbit anti-Fibronectin, and mouse anti-β-actin (Merck, Darmstadt, Germany) were used along with horseradish peroxidase-conjugated secondary antibody (Jackson ImmunoResearch, West Grove, PA, USA). The intensities of each band were quantified using the BioSpectrum^® Imaging System (Analytik Jena US LLC, Upland, CA, USA).