ARPE-19 cells grown on coverslips were fixed with 4% PFA in PBS at 4°C. For immunocytochemistry staining, the ARPE-19 cells on the coverslips were incubated with 5% goat serum in 0.3% Triton X-100 in PBS at RT for 1 h. These cells were then incubated with rabbit anti-IL-22Rα (1:100; abcam, Cambridge, UK) overnight at 4°C. And then, the cells were incubated with Alexa fluor-633 conjugated anti-rabbit Ab (1:2,000; Invitrogen, Carlsbad CA, USA) for 60 min. at RT and finally stained with DAPI. The fixed eye samples were embedded in paraffin and stained with rabbit anti-IL-22Rα (1:200; abcam) and Alexa fluor-633 conjugated anti-rabbit Ab (1:2,000; Invitrogen).
Kim Y., Kim T.W., Park Y.S., Jeong E.M., Lee D.S., Kim I.G., Chung H., Hwang Y.I., Lee W.J., Yu H.G, & Kang J.S. (2016). The Role of Interleukin-22 and Its Receptor in the Development and Pathogenesis of Experimental Autoimmune Uveitis. PLoS ONE, 11(5), e0154904.
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