Log In Sign up
The largest database of trusted experimental protocols

Ab178681

Manufactured by Abcam
Visit Supplier

Ab178681 is a recombinant monoclonal antibody that recognizes the Human PCNA protein. The antibody is intended for use in various immunoassay applications.

Automatically generated - may contain errors

Lab products found in correlation

Ab157808, by Abcam (3 mentions) Ab175772, by Abcam (2 mentions) Sc 374429, by Santa Cruz Biotechnology (2 mentions) Sc 25778, by Santa Cruz Biotechnology (2 mentions) Ab140601, by Abcam (2 mentions) Bml pw8810, by Enzo Life Sciences (2 mentions) β actin, by Cell Signaling Technology (2 mentions) Magna rip rna binding protein immunoprecipitation kit, by Merck Group (1 mentions) Odyssey software, by LI COR (1 mentions)

5 protocols using ab178681

1

RNA-binding protein immunoprecipitation

Check if the same lab product or an alternative is used in the 5 most similar protocols
RIP experiments were performed with the Magna RIP™ RNA-binding protein Immunoprecipitation Kit (Millipore, Stafford, VA, USA) according to the manufacturer's protocol. Anti-Tln1 antibody (1:100, ab157808; Abcam) and anti-PSMC5 antibody (1:50, ab178681; Abcam) were used to coprecipitate RNA. RNA quantity was detected by qRT-PCR.
Li X., Sun Y., Huang S., Chen Y., Chen X., Li M., Si X., He X., Zheng H., Zhong L., Yang Y., Liao W., Liao Y., Chen G, & Bin J. (2018). Inhibition of AZIN2-sv induces neovascularization and improves prognosis after myocardial infarction by blocking ubiquitin-dependent talin1 degradation and activating the Akt pathway. EBioMedicine, 39, 69-82.
+ Open protocol
+ Expand
2

Western Blot Analysis of Cytoskeletal Proteins

Check if the same lab product or an alternative is used in the 5 most similar protocols
Western blot analysis was performed as previously reported [22 (link)]. The primary antibodies used were against Tln1 (1:100, ab157808; Abcam), integrin β1 (ITGB1) (1:200, sc-374429; Santa Cruz) and PSMC5 (1:50, ab178681; Abcam). GAPDH (1:200, sc-25778; Santa Cruz) was used as the loading control. Alexa Fluor 480 (1:10,000, ab175772; Abcam) was used as the secondary antibody. Immunoreactive bands were visualized with Odyssey Software (version 1.2; LI-COR, Lincoln, NE, USA). Protein expression was measured using ImageJ Analysis software (NIH, Bethesda, MD, USA).
Li X., Sun Y., Huang S., Chen Y., Chen X., Li M., Si X., He X., Zheng H., Zhong L., Yang Y., Liao W., Liao Y., Chen G, & Bin J. (2018). Inhibition of AZIN2-sv induces neovascularization and improves prognosis after myocardial infarction by blocking ubiquitin-dependent talin1 degradation and activating the Akt pathway. EBioMedicine, 39, 69-82.
+ Open protocol
+ Expand
3

Polyubiquitin and Proteasomal Subunit Detection

Check if the same lab product or an alternative is used in the 5 most similar protocols
Rabbit monoclonal antibodies included K48 polyubiquitin (1:1000, #ab140601, Abcam, Cambridge, MA), PSMC5/RPT6 (1:5000, #ab178681, Abcam) and β-actin (1:1000, #3450, Cell Signaling Technology). Mouse polyclonal antibodies included total polyubiquitin (1:1000, #BML-PW8810, Enzo Life Sciences, Farmingdale, NY).
McFadden T., Farrell K., Martin K., Musaus M, & Jarome T.J. (2022). Short-term exposure to an obesogenic diet causes dynamic dysregulation of proteasome-mediated protein degradation in the hypothalamus of female rats. Nutritional neuroscience, 26(4), 290-302.
+ Open protocol
+ Expand
4

Western Blot Analysis of Key Proteins

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Western blot analysis was performed as previously reported 29 (link). The primary antibodies used were against MCP-1 (1:100, ab157808; Abcam), VCAM-1 (1:200, sc-374429; Santa Cruz) and MMP-2 (1:50, ab178681; Abcam). β-Actin (1:200, sc-25778; Santa Cruz) was used as a loading control. Alexa Fluor 480 (1:10,000, ab175772; Abcam) was used as the secondary antibody. Immunoreactive bands were visualized with Odyssey Software (version 1.2; LI-COR, Lincoln, NE, USA). Protein expression was measured using ImageJ analysis software (NIH, Bethesda, MD, USA).
Li X., Guo S., Xu T., He X., Sun Y., Chen X., Cao S., Si X., Liao W., Liao Y., Han Y, & Bin J. (2020). Therapeutic ultrasound combined with microbubbles improves atherosclerotic plaque stability by selectively destroying the intraplaque neovasculature. Theranostics, 10(6), 2522-2537.
+ Open protocol
+ Expand
5

Polyubiquitin and Proteasomal Subunit Detection

Check if the same lab product or an alternative is used in the 5 most similar protocols
Rabbit monoclonal antibodies included K48 polyubiquitin (1:1000, #ab140601, Abcam, Cambridge, MA), PSMC5/RPT6 (1:5000, #ab178681, Abcam) and β-actin (1:1000, #3450, Cell Signaling Technology). Mouse polyclonal antibodies included total polyubiquitin (1:1000, #BML-PW8810, Enzo Life Sciences, Farmingdale, NY).
McFadden T., Farrell K., Martin K., Musaus M, & Jarome T.J. (2023). Short-term exposure to an obesogenic diet causes dynamic dysregulation of proteasome-mediated protein degradation in the hypothalamus of female rats. Nutritional neuroscience, 26(4).
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!