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F2 quantikine elisa kit

Manufactured by R&D Systems
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Sourced in United States

The F2 Quantikine ELISA kit is a quantitative sandwich enzyme immunoassay designed for the measurement of F2 (Thrombin) levels in cell culture supernates, serum, and plasma.

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Human lipocalin 2 ngal quantikine elisa kit, by R&D Systems (2 mentions) Human il 8 cxcl8 quantikine elisa kit, by R&D Systems (2 mentions) Elisa kit, by R&D Systems (2 mentions) Human il 1β, by R&D Systems (1 mentions) Infinite m nano microplate reader, by Tecan (1 mentions)

2 protocols using f2 quantikine elisa kit

1

Biomarker Analysis Using ELISA Kits

Cited 1 time
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The biomarker analysis was performed using ELISA kits purchased from R&D Systems/Bio-Techne (Minneapolis, MN, USA), including human Lipocalin-2/NGAL Quantikine ELISA Kit (Catalog number SLCN20), human IL-8/CXCL8 Quantikine ELISA Kit (Catalog number S8000C), and human IL-1β/IL-1F2 Quantikine ELISA kit (Catalog number SLB50), as per the manufacturer’s instructions. Biomarker “positivity” was determined using thresholds presented previously in the literature (NGAL ≥38.0 ng/ml, IL-8 ≥20.6 pg/ml, and IL-1β ≥12.4 pg/ml) [18] (link), [19] (link). For this analysis, “consensus” was defined as two or more biomarkers meeting the positivity threshold.
Haley E., Luke N., Mathur M., Festa R.A., Wang J., Jiang Y., Anderson L, & Baunoch D. (2023). Comparison Shows that Multiplex Polymerase Chain Reaction Identifies Infection-associated Urinary Biomarker–positive Urinary Tract Infections That Are Missed by Standard Urine Culture. European Urology Open Science, 58, 73-81.
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2

Measurement of Urine Inflammatory Biomarkers

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Urine levels of NGAL, IL-8, and IL-1β were analyzed according to the manufacturer’s instructions, using ELISA kits from R&D Systems/Bio-Techne (Minneapolis, MN), including human Lipocalin-2 / NGAL Quantikine ELISA Kit (Catalog number SLCN20), human IL-8 / CXCL8 Quantikine ELISA Kit (Catalog number S8000C), and human IL-1β / IL-1F2 Quantikine ELISA kit (Catalog number SLB50). OD readings at 450 and 540 nm, respectively, were measured on an Infinite M Nano + microplate reader (TECAN, Switzerland).
Akhlaghpour M., Haley E., Parnell L., Luke N., Mathur M., Festa R.A., Percaccio M., Magallon J., Remedios-Chan M., Rosas A., Wang J., Jiang Y., Anderson L, & Baunoch D. (2024). Urine biomarkers individually and as a consensus model show high sensitivity and specificity for detecting UTIs. BMC Infectious Diseases, 24, 153.
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