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Megascript t7 amplification kit

Manufactured by Thermo Fisher Scientific
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Sourced in United States

The MegaScript T7 Amplification Kit is a tool designed for in vitro transcription of RNA from DNA templates. It provides a simple and efficient method to generate high yields of RNA from a DNA template containing a T7 RNA polymerase promoter.

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Lab products found in correlation

Genechip sample cleanup module kit, by Thermo Fisher Scientific (1 mentions) Ovation pico rna amplification system, by Tecan (1 mentions)

Spelling variants of this product

MEGAscript T7 kit (540 citations) MEGAscript kit (251 citations) MEGAscript (96 citations) Megascript T7 (53 citations) T7 kits (3 citations)

2 protocols using megascript t7 amplification kit

1

RNA Amplification for Microarray Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
An ovation Pico RNA Amplification System (Nugen Technologies) was used for the amplification of RNA destined for microarray analysis. RNA amplification of LCM ARC samples used to validate genes identified by microarray analysis was performed using a MegaScript T7 Amplification Kit (Ambion) in combination with the GeneChip sample CleanUp Module kit (Affymetrix). The use of a different method of RNA amplification enhanced the validation of the microarray data.
Martin-Gronert M.S., Stocker C.J., Wargent E.T., Cripps R.L., Garfield A.S., Jovanovic Z., D'Agostino G., Yeo G.S., Cawthorne M.A., Arch J.R., Heisler L.K, & Ozanne S.E. (2016). 5-HT2A and 5-HT2C receptors as hypothalamic targets of developmental programming in male rats. Disease Models & Mechanisms, 9(4), 401-412.
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2

RNA Amplification for Microarray Validation

Check if the same lab product or an alternative is used in the 5 most similar protocols
Ovation Pico RNA Amplification System (Nugen Technologies Inc, San Carlos, California, USA) was used for the amplification of RNA destined for microarray analysis. RNA amplification of LCM ARC samples (n=6 Control and n=6 PLP each from a different litter) used to validate genes identified by microarray analysis was performed using a MegaScript T7 Amplification Kit (Ambion) in combination with the GeneChip sample CleanUp Module kit (Affymetrix Inc, Santa Clara, California, USA). The use of a different method of RNA amplification enhanced the validation of the microarray data.
Wargent E.T., Martin-Gronert M.S., Cripps R.L., Heisler L.K., Yeo G.S.H., Ozanne S.E., Arch J.R.S, & Stocker C.J. (2020). Developmental programming of appetite and growth in male rats increases hypothalamic serotonin (5-HT)5A receptor expression and sensitivity. International journal of obesity (2005), 44(9).
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