The periodic acid-Schiff (PAS) staining was performed using the PAS staining kit (#395B-1KT; Sigma-Aldrich, St. Louis, MO, USA). For immunohistochemistry, human kidney sections were stained with the primary antibodies against DKK1 (sc-25516; Santa Cruz, Dallas, TX, USA), fibronectin (ab45688; Abcam, Cambridge, UK) or TGF-β1 (BS1361; Bioworld Technology, Dublin, OH, USA), whereas rat kidney sections were stained with antibodies against DKK1 (sc-25516; Santa Cruz, Dallas, TX, USA), TGF-β1 (E11264; Spring Bioscience, Pleasanton, CA, USA), collagen IV (BSB5355; BioSB, Santa Barbara, CA, USA), fibronectin (BS1644; Bioworld Technology, Dublin, OH, USA) or IL-1β (sc-7884; Santa Cruz, Dallas, TX, USA). For quantification of PAS and immunohistochemical staining, six areas from each section were analyzed using the Image-Pro Plus software (Media Cybernetics, Silver Spring, MD, Version 6.3). Immunofluorescence staining of rat kidney sections with the β-catenin antibody (GTX101435; GeneTex, Irvine, CA, USA) was conducted according to the previously described procedure [49 (link)]. The mean fluorescence intensity of β-catenin in kidney sections was quantified using the CellSens software package Version 2.1 (Olympus Medical Systems, Tokyo, Japan).
Bs1361
Manufactured by Bioworld Technology
Sourced in United Kingdom
The BS1361 is a laboratory-grade centrifuge designed for basic separation tasks. It features a compact and durable construction, suitable for a variety of laboratory environments. The centrifuge can accommodate sample tubes of various sizes and shapes, allowing for efficient separation of biological and chemical samples.
Automatically generated - may contain errors
Lab products found in correlation
Ab45688, by Abcam (1 mentions)
Pas staining kit, by Merck Group (1 mentions)
Sc 7884, by Santa Cruz Biotechnology (1 mentions)
Sc 25516, by Santa Cruz Biotechnology (1 mentions)
Image pro plus software, by Media Cybernetics (1 mentions)
Bs70000, by Bioworld Technology (1 mentions)
Alexa fluor 488 conjugated goat anti rabbit igg, by Merck Group (1 mentions)
Sc 5275, by Santa Cruz Biotechnology (1 mentions)
Lsm780 confocal laser scanning microscope, by Zeiss (1 mentions)
2 protocols using bs1361
1
Histological and Immunostaining Analysis of Kidney Tissue
2
Immunostaining of Frozen Penile Tissue
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Frozen penile tissue serial coronal sections (30 µm) were incubated overnight at 4 ℃ with diluted primary antibodies including: TGF-β1 (1:200; BS1361; Bioworld Technology), CTGF (1:200; BS7445; Bioworld Technology), α-SMA (1:200; BS70000; Bioworld Technology) and CD63 (1:500, SC-5275, Santa Cruz Biotechnology) followed by the Alexa Fluor488-conjugated goat anti-rabbit IgG or Alexa Fluor594-conjugated goat anti-mouse IgG secondary antibody (all 1:200, Sigma-Aldrich). Images were captured with use of a LSM780 laser scanning confocal microscope (ZEISS, Germany). A minimum of 6 images were taken from each animal for analysis by Image-Pro plus 6.0 software.
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