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α phospho s139 h2ax s139

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α-phospho-S139-H2AX (S139) is a laboratory reagent used in the detection and quantification of phosphorylated histone H2AX at serine 139. This modification is a marker of DNA double-strand breaks and cellular response to DNA damage.

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Lab products found in correlation

Goat anti rabbit igg h l hrp, by Thermo Fisher Scientific (2 mentions) Goat anti mouse igg h l hrp, by Thermo Fisher Scientific (2 mentions) α brdu, by BD (2 mentions) Normal mouse igg, by Merck Group (2 mentions) α macroh2a1, by Merck Group (2 mentions) α h2ax, by Abcam (2 mentions) α h2b, by Abcam (2 mentions) α brca1, by Santa Cruz Biotechnology (2 mentions) α gapdh, by Santa Cruz Biotechnology (2 mentions) α γh2ax, by Merck Group (2 mentions) α phospho s139 h2ax, by Merck Group (2 mentions) α h2a, by Abcam (2 mentions) Img 124a, by Merck Group (2 mentions) α atrx, by Cell Signaling Technology (2 mentions) Img 124a, by Novus Biologicals (2 mentions) Alexa fluor 488 or 647, by Thermo Fisher Scientific (2 mentions)

Close spelling variants of this product

α-phospho-S139-H2AX Phospho-S139-H2AX α-phospho-H2AX Phospho-H2AX

2 protocols using α phospho s139 h2ax s139

1

ChIP-Seq and Western Blot Antibodies

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The following antibodies were used for ChIP: α-macroH2A1.2 (Millipore MABE61), α-phospho-S139-H2AX (Millipore 05–636), α-H2B (Abcam ab52484), α-H2A (Abcam ab18255), TRF2 (Novus Biologicals IMG-124A) and normal mouse IgG (Millipore 12–371). Primary antibodies for western blotting were: α-macroH2A1.2 (Millipore MABE61), α-phospho-S139-H2AX (S139) (Millipore 05–636), α-BRCA1 (Santa Cruz sc-6954), α-ATRX (Cell signaling 14820s), α-GAPDH (Santa Cruz sc-32233), α-H2AX (Abcam ab20669). Secondary antibodies were goat anti-mouse IgG (H+L)-HRP (Invitrogen 31430) and goat anti-rabbit IgG (H+L)-HRP (Invitrogen 31460). Primary antibodies for IF were α-BRCA1 (Santa Cruz sc-6954), α-γ-H2AX (Millipore 05–636, Abcam ab11174), α-BrdU (BD Biosciences 555627), and TRF2 (Novus Biologicals IMG-124A), secondary antibodies were goat-anti-mouse or goat-anti-rabbit IgG (H+L) coupled to Alexa Fluor 488 or 647 (Life Technologies).
Kim J., Sun C., Tran A.D., Chin P.J., Ruiz P.D., Wang K., Gibbons R.J., Gamble M.J., Liu Y, & Oberdoerffer P. (2019). The macroH2A1.2 histone variant links ATRX loss to alternative telomere lengthening.. Nature structural & molecular biology, 26(3), 213-219.
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2

ChIP-Seq and Western Blot Antibodies

Check if the same lab product or an alternative is used in the 5 most similar protocols
The following antibodies were used for ChIP: α-macroH2A1.2 (Millipore MABE61), α-phospho-S139-H2AX (Millipore 05–636), α-H2B (Abcam ab52484), α-H2A (Abcam ab18255), TRF2 (Novus Biologicals IMG-124A) and normal mouse IgG (Millipore 12–371). Primary antibodies for western blotting were: α-macroH2A1.2 (Millipore MABE61), α-phospho-S139-H2AX (S139) (Millipore 05–636), α-BRCA1 (Santa Cruz sc-6954), α-ATRX (Cell signaling 14820s), α-GAPDH (Santa Cruz sc-32233), α-H2AX (Abcam ab20669). Secondary antibodies were goat anti-mouse IgG (H+L)-HRP (Invitrogen 31430) and goat anti-rabbit IgG (H+L)-HRP (Invitrogen 31460). Primary antibodies for IF were α-BRCA1 (Santa Cruz sc-6954), α-γ-H2AX (Millipore 05–636, Abcam ab11174), α-BrdU (BD Biosciences 555627), and TRF2 (Novus Biologicals IMG-124A), secondary antibodies were goat-anti-mouse or goat-anti-rabbit IgG (H+L) coupled to Alexa Fluor 488 or 647 (Life Technologies).
Kim J., Sun C., Tran A.D., Chin P.J., Ruiz P.D., Wang K., Gibbons R.J., Gamble M.J., Liu Y, & Oberdoerffer P. (2019). The macroH2A1.2 histone variant links ATRX loss to alternative telomere lengthening.. Nature structural & molecular biology, 26(3), 213-219.
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