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Anti cep192

Manufactured by Merck Group
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Anti-Cep192 is a laboratory equipment product developed by Merck Group. It is a tool used for the detection and analysis of the Cep192 protein, which plays a crucial role in cellular processes. The core function of Anti-Cep192 is to provide researchers and scientists with the means to study and understand the role of the Cep192 protein in various biological systems.

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Lab products found in correlation

Anti p53, by Cell Signaling Technology (2 mentions) Anti rac1, by BD (2 mentions) Anti α tubulin dm1a, by Merck Group (2 mentions) E cadherin, by Thermo Fisher Scientific (2 mentions) Anti mcak, by Fortis Life Sciences (2 mentions) Phospho ser15, by Cell Signaling Technology (2 mentions) Anti p p53, by Cell Signaling Technology (2 mentions) Imagequant las 4000, by GE Healthcare (2 mentions)

2 protocols using anti cep192

1

Immunoblotting analysis of cell proteins

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cells were collected and ressuspended in Laemmli buffer and proteins were separated on sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and transferred onto PVDF membranes. Antibodies used included anti α-tubulin DM1a (1:2000; Sigma), anti-Cep192 (1:1000; generous gift from L. Pelletier), anti-MCAK (1:1000; Bethyl Laboratories), anti-p53 (1:1000, Cell Signaling), anti-P-p53 (1:1000, phospho-Ser15, Cell Signaling), E-cadherin (1:1000, Invitrogen) and anti-Rac1 (1:1000, BD Biosciences). Images were acquired using ImageQuant LAS4000 (GE Healthcare) and when required band intensity was quantified using ImageJ.
Godinho S.A., Picone R., Burute M., Dagher R., Su Y., Leung C.T., Polyak K., Brugge J.S., Thery M, & Pellman D. (2014). Oncogene-like induction of cellular invasion from centrosome amplification. Nature, 510(7503), 167-171.
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2

Immunoblotting analysis of cell proteins

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cells were collected and ressuspended in Laemmli buffer and proteins were separated on sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and transferred onto PVDF membranes. Antibodies used included anti α-tubulin DM1a (1:2000; Sigma), anti-Cep192 (1:1000; generous gift from L. Pelletier), anti-MCAK (1:1000; Bethyl Laboratories), anti-p53 (1:1000, Cell Signaling), anti-P-p53 (1:1000, phospho-Ser15, Cell Signaling), E-cadherin (1:1000, Invitrogen) and anti-Rac1 (1:1000, BD Biosciences). Images were acquired using ImageQuant LAS4000 (GE Healthcare) and when required band intensity was quantified using ImageJ.
Godinho S.A., Picone R., Burute M., Dagher R., Su Y., Leung C.T., Polyak K., Brugge J.S., Thery M, & Pellman D. (2014). Oncogene-like induction of cellular invasion from centrosome amplification. Nature, 510(7503), 167-171.
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