Cell staining with PE anti-human Siglec-8 (BioLegend), PE anti-human CD (CD80) (BioLegend), APC anti-human FcεR1α (BioLegend), APC anti-human CD86 (BioLegend), APC anti-human CD23 (BioLegend), APC anti-human CD63 (BioLegend), PE anti-human PDL1 (BioLegend), PE anti-human IL-5 receptor alpha (IL-5Rα; R&D Systems) were assessed by flow cytometry on an Accuri C6 flow cytometer (BD Biosciences, Franklin Lakes, NJ, USA) and data analyzed with FlowJo software (Tree Star Inc., Ashland, OR, USA).
Pe anti human pdl1
Manufactured by BioLegend
Sourced in United States
PE anti-human PDL1 is a fluorescent-labeled antibody that binds to the programmed death-ligand 1 (PD-L1) protein expressed on human cells. It can be used for the detection and analysis of PD-L1 expression in various applications, such as flow cytometry.
Automatically generated - may contain errors
Lab products found in correlation
Apc anti human cd86, by BioLegend (1 mentions)
Apc anti human cd63, by BioLegend (1 mentions)
Apc antihuman cd23, by BioLegend (1 mentions)
Apc antihuman fcεr1α, by BioLegend (1 mentions)
Accuri c6 flow cytometer, by BD (1 mentions)
Fitc anti human cd63, by BioLegend (1 mentions)
Cytoflex, by Beckman Coulter (1 mentions)
Cell stimulation cocktail plus protein transport inhibitor, by Thermo Fisher Scientific (1 mentions)
3 protocols using pe anti human pdl1
1
Multiparameter Flow Cytometry Immunophenotyping
2
Exosome Characterization by Flow Cytometry
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Exosomes were resuspended in 100 ul PBS and then added 10 ul aldehyde/sulfate beads (Invitrogen, Catalog #A37304) into it, rotated at room temperature for 15 min. We added 600 ul PBS and rotated at 4°C overnight and 400 ul 1 M glycine rotated at room temperature for 30 min. Samples were spined at 12,000 rpm for 1.5 min and aspirated supernatant, resuspended precipitate in 100 ul 10% BSA, rotating at room temperature for 45 min. Specimens staining was performed for 30 min at 4°C with the following antibodies: PE Anti-Human-PD-L1 (BioLegend, Catalog #329706), Alexa Fluor® 647 anti-human CD279 (PD-1) Antibody (BioLegend, Catalog # 329910), PerCP/Cyanine 5.5 anti-human CD9 Antibody (BioLegend, Catalog #312110) and FITC anti-human CD63(BioLegend, Catalog #329906) tested in Beckman Coulter CytoFLEX (CA, USA).
3
Quantifying Immune Checkpoint Markers
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To assess the expression of PD-L1 and major histocompatibility complex (MHC) molecules, tumor cells were treated as described above in the cell cycle analysis. The markers of human and mouse tumor cells, as well as those of mouse T cells, were determined using flow cytometry after staining with specific antibodies conjugated with different fluorescent molecules. For intracellular staining of interferon (IFN)-γ, erythrocyte-excluded spleen cells were first incubated with a cell stimulation cocktail plus protein transport inhibitors (eBioscience; Cat No. 4975-03) at 37°C under 5% CO 2 for 5 hour. The following antibodies were used: PE-anti-human PD-L1 (Biolegend; Cat
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