The tumor tissue was made into paraffin sections as described previously. After deparaffinization, the sections were permeated with 0.1% Triton X-100, and blocked with 3% H2O2 at room temperature. Then the sections were incubated with TUNEL buffer (Roche, Basel, Switzerland) for 60 min at 37 °C in the dark, and incubated with Converter-POD reagent for 30 min at 37 °C. Subsequently, the sections were reacted with DAB substrate (Solarbio), and counterstained with hematoxylin. Finally, the sections were dehydrated, mounted and photographed with a microscope (BX53, Olympus) at 400× magnification.
Tunel buffer
Manufactured by Roche
Sourced in Switzerland
TUNEL buffer is a reagent used in the TUNEL (Terminal deoxynucleotidyl transferase dUTP Nick End Labeling) assay. The TUNEL assay is a method used to detect and quantify apoptosis, or programmed cell death, in cells. The TUNEL buffer provides the necessary components for the TUNEL reaction, which labels the DNA strand breaks that occur during apoptosis.
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Lab products found in correlation
4 protocols using tunel buffer
1
TUNEL Assay for Apoptosis Detection
2
TUNEL Assay for Apoptosis Detection
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Prepared pancreatic slides (4 M) were dewaxed with xylene twice, washed with phosphate-buffered saline solution (PBS) twice, then dehydrated with gradient alcohol (5 min). Subsequently, 0.3% Trixon-X100 was used to penetrate cells at room temperature (5 min). Following two washes with PBS, apoptotic cells were stained with TUNEL buffer (Roche Diagnostics GmbH, Roche, Germany) for 1 h at 37°C. The nuclei were stained using Antifade Mounting Medium with 4,6-diamidino-2-phenylindole (DAPI) (Sigma, United Kingdom), and the cells were mounted. The photos were captured using an inverted fluorescent microscope.
3
TUNEL Assay for Apoptosis Detection
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The tumor tissue was made into para n sections as described previously. After depara nization, the sections were permeated with 0.1% Triton X-100, and blocked with 3% H 2 O 2 at room temperature. Then the sections were incubated with TUNEL buffer (Roche, Basel, Switzerland) for 60 min at 37 ℃ in the dark, and incubated with Converter-POD reagent for 30 min at 37 ℃. Subsequently, the sections were reacted with DAB substrate (Solarbio), and counterstained with hematoxylin. Finally, the sections were dehydrated, mounted and photographed with a microscope at 400× magni cation.
4
TUNEL Assay for Apoptosis Detection
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The tumor tissue was made into para n sections as described previously. After depara nization, the sections were permeated with 0.1% Triton X-100, and blocked with 3% H 2 O 2 at room temperature. Then the sections were incubated with TUNEL buffer (Roche, Basel, Switzerland) for 60 min at 37 ℃ in the dark, and incubated with Converter-POD reagent for 30 min at 37 ℃. Subsequently, the sections were reacted with DAB substrate (Solarbio), and counterstained with hematoxylin. Finally, the sections were dehydrated, mounted and photographed with a microscope at 400× magni cation.
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