Streptavidin horseradish peroxidase hrp

Manufactured by Cell Signaling Technology

Sourced in Japan

Streptavidin horseradish peroxidase (HRP) is a conjugate of streptavidin, a protein that binds to biotin, and horseradish peroxidase, an enzyme that catalyzes a color-producing reaction. This product is used in various immunoassay and detection techniques that involve biotin-avidin interactions.

Automatically generated - may contain errors

Lab products found in correlation

Cycloheximide (chx), by Enzo Life Sciences (1 mentions) Doxycycline, by Thermo Fisher Scientific (1 mentions) Torin1, by Bio-Techne (1 mentions) Mouse anti flag m2, by Merck Group (1 mentions) Mk 2206, by Selleck Chemicals (1 mentions) Rapamycin, by Merck Group (1 mentions) Phorbol myristate acetate (pma), by Merck Group (1 mentions) β actin antibody, by Cell Signaling Technology (1 mentions) Annexin 5 binding buffer, by BioLegend (1 mentions) Ambion silencer select pre designed sirna, by Thermo Fisher Scientific (1 mentions) Anti rabbit igg hrp linked antibody, by Cell Signaling Technology (1 mentions) Propidium iodide, by Merck Group (1 mentions)

2 protocols using streptavidin horseradish peroxidase hrp

Characterization of Cellular Signaling Pathways

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The following inhibitors/chemicals and antibodies were used in this study.
Cycloheximide (Enzo Life Sciences, 380-269-G005), PMA (EMD Millipore; 524400), Torin 1 (Tocris Bioscience; 4247), rapamycin (EMD Millipore; 553212), RSKi/BI-D1870 (Selleck Chemicals; S2843), MK-2206 (Selleck Chemicals; S1078), doxycycline (Fisher Bioreagents; BP26535), biotin phenol/biotinyl tyramide (Adipogen; CDX-B0270), and biotin (Sigma; B4639).
Monoclonal anti-HA-agarose mouse (Sigma; A2095), streptavidin agarose resin (Pierce; 20347), anti-FLAG M2 mouse (Sigma; F3165), streptavidin horseradish peroxidase (HRP) (Cell Signaling Technologies [CST]; 3999), pERK1/2 (CST; 4370), pRSK1 (CST; 9341), RSK1 (CST; 8408), RSK1/2/3 (CST; 14813, pAKT1 (CST; 4060), pS6K (CST; 9234), pS6 (CST; 8585), p4EBP1 (CST; 2855), myc (CST: 2278), tubulin (CST; 3873), TSC2 (Santa Crux; sc271314), pTSC2 (Santa Cruz; sc293149), HA (BioLegend; MMS-101P), Ltn1/RNF160 (Abcam; ab104375), RPS3 (Bethyl; A303-840A), RPL7 (Bethyl; A300-741A), and TTLL1 (Thermo Fisher; PA5-27285).

Zuzow N., Ghosh A., Leonard M., Liao J., Yang B, & Bennett E.J. (2018). Mapping the mammalian ribosome quality control complex interactome using proximity labeling approaches. Molecular Biology of the Cell, 29(10), 1258-1269.

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Annexin V-Based Apoptosis Analysis

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Annexin V Binding Buffer and FITC-linked annexin V (annexin V-FITC) was purchased from BioLegend (San Diego, CA, USA). Propidium iodide (PI) was purchased from Sigma-Aldrich (St. Louis, MO, USA). Anti-human phospho-IRF3 antibody (#37829), anti-human cGAS (#D1D3G) antibody (Biotinylated) (#66546), anti-human p21 antibody (#2947), β-actin antibody (#4967), Streptavidin-horseradish peroxidase (HRP) (#3999), anti-rabbit IgG HRP-linked antibody (#7074), and anti-mouse IgG HRP-linked antibody (#7076) were purchased from Cell Signaling Technology Japan, K.K. (Tokyo, Japan). ISD Control/LyoVec^TM and ISD/LyoVec^TM were purchased from InvivoGen (San Diego, CA, USA). Ambion Silencer^® Select pre-designed siRNA against the gene encoding cGAS (ID: s41748), the gene encoding CDKN1A (p21) (ID: s416), and Silencer^® Select Negative #1 Control siRNA (ID: 4390843) were purchased from Invitrogen (Thermo Fisher Scientific, Inc., Waltham, MA, USA).

Nyui T., Yoshino H., Nunota T., Sato Y, & Tsuruga E. (2022). cGAS Regulates the Radioresistance of Human Head and Neck Squamous Cell Carcinoma Cells. Cells, 11(9), 1434.

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