Dulbecco s modified eagle s medium high glucose powder dmem

Sephadex LH-20 (Merck KGaA, Darmstadt, Germany), silica gel 60 (Merck KGaA) and Geduran Si 60 (Merck KGaA) were used for column chromatography. TLC plates (Silica Kiesel 60 F254) were from Merck KGaA. Jasco V-530 ultraviolet spectrophotometer (Jasco International Co., Ltd, Tokyo, Japan) was used to measure UV spectra. IR spectra were obtained on an FT-IR-4100 Jasco spectrophotometer (Jasco). Optical rotations were achieved by a Jasco P-2000 digital polarimeter (Jasco). NMR spectra were obtained by JEOL JNM ECS 400 MHz. Electrospray ionization mass spectrometry (ESIMS) data were collected on a Waters micromass ZQ mass spectrometer (Waters Corporation, Milford, MA, USA). High-resolution ESIMS data was accomplished by a Bruker APEX II spectrometer (FT-ICR/MS, FTMS) (Bruker Daltonics Inc., Billerica, MA, USA). Dulbecco’s modified Eagle’s medium (high glucose) powder (DMEM), 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), p-nitrophenyl-N-acetyl-d-glucosaminide (p-NAG), penicillin and streptomycin, dexamethasone, calcium ionophore A23187, and dimethyl sulfoxide (DMSO) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Fetal bovine serum (FBS) was obtained from Hyclone (Logan, UT, USA). Mouse anti-DNP IgE antibody was a generous gift from Dr. Daniel H. Conrad (Virginia Commonwealth University, Richmond, VA, USA).

Glibenclamide (GLB), streptozotocin (STZ), [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] (MTT), Dulbecco’s modified Eagle’s medium-high glucose powder (DMEM), streptomycin, calcium ionophore A23187, o-phthalaldehyde, p-nitrophenyl-N-acetyl-d-glucosaminide (p-NAG), dexamethasone, and penicillin were bought from Sigma-Aldrich (St. Louis, MO, USA). Roche Diagnostics Germany (Mannheim, Germany) kindly supplied glucotest strips. The radioimmunoassay kit used for determination of insulin level and the glucose oxidase kit were purchased from Amersham Biosciences; (Piscataway, NJ, USA) and Siemens Healthcare Diagnostics (Deerfield, IL, USA), respectively. DNP-BSA (dinitrophenyl-conjugated bovine serum albumin) and FBS (fetal bovine serum) were obtained from Merck (Kenilworth, NJ, USA) and Hyclone (Logan, UT, USA), respectively. Other kits used for assessment of LPO (lipid peroxidation) and MDA (malondialdehyde) were obtained from Fluka (Buchs, Switzerland). Dr. Daniel H. Conrad (Virginia Commonwealth University, Richmond, VA, USA) generously supplied Mouse anti-DNP IgE antibody. All other solvents for LC/MS as well as GLC/MS analyses were of analytical grade.