Isoflo vet

Manufactured by Zoetis

IsoFlo vet is an inhalation anesthetic agent used in veterinary medicine. It is a colorless, volatile liquid that provides general anesthesia when administered to animals. The product's core function is to induce and maintain a state of unconsciousness in animals during medical procedures.

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IsoFlo (34 citations)

2 protocols using isoflo vet

Testosterone Replacement in Castrated Mice

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Mice were anesthetized with isoflurane (IsoFlo vet, Vnr 002185, Zoetis) and either bilaterally castrated (orchiectomized; ORX) or sham-castrated (Sham). Buprenorphine (Temgesic, RB Pharmaceuticals Ltd) was used for analgesia after all surgical procedures. Following castration, some mice were subcutaneously injected with vehicle (pure corn oil, catalog No. C8267, Sigma) or a physiological dose [11 (link)] of testosterone propionate (3 mg/kg/day; catalog No. 86541, Sigma) every 3 days for 3 weeks. A working solution of testosterone was made by dissolving 45 mg of testosterone propionate in 0.4 mL of 100% ethanol and then adding 30 mL of pure corn oil. Success of castration and testosterone replacement was verified by recording the weight of the androgen-sensitive seminal vesicles at the study end.

Svedlund Eriksson E., Johansson I., Mårtensson A.K., Lantero Rodriguez M., Schilperoort M., Kroon J., Kooijman S., Omerovic E., Andersson L., Levin M.C., Rensen P.C, & Tivesten Å. (2022). Castration of Male Mice Induces Metabolic Remodeling of the Heart. Journal of the Endocrine Society, 6(11), bvac132.

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Surgical Castration in Mice

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The mice were anesthetized with isoflurane (IsoFlo vet, Vnr 002185; Zoetis) and either sham operated or bilaterally castrated/orchiectomized. Buprenorphine (Temgesic; RB Pharmaceuticals, Ltd) was used for analgesia after all surgical procedures.

Wilhelmson A.S., Lantero Rodriguez M., Svedlund Eriksson E., Johansson I., Fogelstrand P., Stubelius A., Lindgren S., Fagman J.B., Hansson G.K., Carlsten H., Karlsson M.C., Ekwall O, & Tivesten Å. (2018). Testosterone Protects Against Atherosclerosis in Male Mice by Targeting Thymic Epithelial Cells—Brief Report. Arteriosclerosis, Thrombosis, and Vascular Biology, 38(7), 1519-1527.

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