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Anti argonaute 2 anti ago2

Manufactured by Merck Group
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Anti-Argonaute-2 (anti-ago2) is a laboratory reagent used in molecular biology and biochemistry research. It is an antibody that specifically binds to the Argonaute-2 (Ago2) protein, which is a key component of the RNA-induced silencing complex (RISC). The primary function of this product is to facilitate the detection and quantification of Ago2 in various experimental systems, such as cell lysates or tissue samples.

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Lab products found in correlation

Magna rip rna binding protein immunoprecipitation kit, by Merck Group (2 mentions) Anti immunoglobulin g anti igg, by Merck Group (2 mentions) Rip buffer, by Merck Group (1 mentions) Anti ago2, by Merck Group (1 mentions)

Close spelling variants of this product

Anti-Ago2 (91 citations) Anti-human argonaute 2 (Ago2) antibodies (5 citations)

2 protocols using anti argonaute 2 anti ago2

1

RIP Assay for LINC00659 and miR-760 Interactions

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RNA immunoprecipitation (RIP) assay was performed using the Magna RIP RNA-Binding Protein Immunoprecipitation Kit (Millipore), anti-Immunoglobulin G (anti-IgG; Millipore) and anti-Argonaute-2 (anti-ago2; Millipore). SW620 and HT29 cells after transfection for 48 h were lysed in RIP buffer (Millipore), and proteins were incubated with magnetic beads conjugated with anti-ago2, and anti-IgG served as a control. qRT-PCR was conducted to detect the abundance of LINC00659 and miR-760.
Li J., Qin Y., Wang W., Yang K, & Zhang M. (2021). Peiminine inhibits the progression of colorectal cancer through up-regulating miR-760 via declining the expression of long noncoding RNA LINC00659. Anti-cancer drugs, 32(2).
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2

Confirmation of circRNA-miRNA Interaction

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RIP experiment was employed to confirm the binding relation between circ_0004104 and miR-328-3p with Magna RIP™ RNA-Binding Protein Immunoprecipitation Kit (Millipore). Cell extracts were prepared using RIP lysis buffer, and anti-Argonaute2 (anti-Ago2; Millipore) or anti-Immunoglobulin G (anti-IgG; Millipore)-pre-coated magnetic beads were incubated with cell lysates. The levels of enriched RNAs were measured by RT-qPCR.
Zhang C., Wang L, & Shen Y. (2021). Circ_0004104 knockdown alleviates oxidized low-density lipoprotein-induced dysfunction in vascular endothelial cells through targeting miR-328-3p/TRIM14 axis in atherosclerosis. BMC Cardiovascular Disorders, 21, 207.
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