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2 protocols using anti mouse cd31 2502 antibody

1

Vinorelbine and Everolimus Combination Therapy

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Vinorelbine (Navelbine®) (10 mg/mL) was obtained from Pierre Fabre Medicament (Boulogne, France) and was dissolved in PBS to a final concentration of 0.375 mg/mL before use. Everolimus (RAD001) was obtained from Novartis (Basel, Switzerland) and dissolved in a vehicle (30% Captisol® in water) to achieve the appropriate concentration for the treatments.
Antibodies against AKT (#9272), p70S6K (#9202), survivin (#2803), S6R (#2217), Rb (#9313), Cyclin B1 (#4138), eIF4E (#9742), Cdc25C (#4688), cleaved caspase 3 (#9661), cleaved caspase 7 (#9491), cleaved PARP (#5625), Cyclin D1 (#2978), Cdc2 (#9112), and α-Tubulin (#2144) and phosphorylation-specific antibodies against RB Ser807/811 (#9308), AKT Ser473 (#9271), mTOR Ser2448 (#5536), p70S6K Thr421/424 (#9204), S6R Ser235/236 (#4858), 4EBP1 Thr70 (#9455), Histone H3 Ser10 (#9701), Cyclin D1 Thr286 (#3300), Cdc25C Ser216 (#4901), Cdc2 Tyr15 (#9111), eIF4E Ser209 (#9741), and ERK1/2 Thr202/Tyr204 (#4370) were obtained from Cell Signaling Technology (Beverly, MA, USA). RKIP (#37-2100) was obtained from Invitrogen. p-Cdk2 Thr14/Tyr15 (sc-28435-R), ERK1/2 (sc-94), and p27 (sc-528) were obtained from Santa Cruz Biotechnology Inc. (Santa Cruz, CA, USA). Anti-mouse CD31 (#2502) antibody was purchased from BioLegend (San Diego, CA, USA).
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2

Antibody Panel for Cancer Signaling

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Antibodies against AKT (#9272), FGFR-3 (#4574), FGFR-4 (#8562), p70S6K (#9202), Survivin (#2803), Cdk2 (#2546), Cdc25C (#4688), Cyclin B1 (#4138), cleaved caspase 3 (#9661), cleaved PARP (#5625), Cdc2 (#9112), Rb (#9313), S6R (#2217), 4EBP1 (#9452) and α-tubulin (#2144) were obtained from Cell Signaling Technology, Beverly, MA, USA. Phosphorylation-specific antibodies against AKT Ser473 (#9271), FRS2-α Tyr439 (#3861), p70S6K Thr421/424 (#9204), S6R Ser235./236 (#2211), 4EBP1 Thr70 (#9455), Histone 3 Ser10 (#9701), Cdc2 Tyr15 (#9111), p-mTOR (#2971), p-Rb (#9308) and ERK1/2 Thr202/Tyr204 (#4370) were also obtained from Cell Signaling Technology, Beverly, MA, USA. The antibodies against FGFR-2 (sc-122), ERK1/2 (sc-94), FRS2-α (sc-17841), E2F1 (sc-251) and p27 (sc-528) were obtained from Santa Cruz Biotechnology Inc, Santa Cruz, CA, USA. The antibody LYVE-1 (AB-2988) was obtained from EMD Millipore, Billerica, MA, USA. The anti-mouse CD31 (#2502) antibody was obtained from BioLegend, San Diego, CA, USA. Infigratinib and Sorafenib were purchased from Selleck Chemicals, Houston, TX, USA. Bevacizumab (Avastin) was from Genentech, Inc., South San Francisco, CA, USA.
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