Pymol software version 4

An automated homology model was constructed using SWISS-MODEL (Swiss Institute of Bioinformatics, BIOZENTRUM, University of Basel, Switzerland) for the consensus RPO30 gene sequences of the SPPV and GTPV isolates. The homology model for all SPPV and GTPV isolates of the RPO30 protein was predicted and proteins were matched from ₁M to ₂₀₄K of the E4L protein of VACV crystallized structure (6RIC_S), which has 259 amino acid residues. Further, the automated structural models were validated using RAMPAGE software (https://www.ccp4.ac.uk/html/rampage.html).
All modeled structures were generated using the X-ray crystallographic structure of the E4L protein (PDB ID: 6RIC_S) of the VACV (₁M to ₂₀₄K) as a reference and visualized using PyMol software version 4.6.0 (Schrödinger, Inc., New York, USA). Structural validation was performed using the Structural Analysis and Verification (SAVES) server (https://saves.mbi.ucla.edu/). Energy minimization and refinement were performed using the ModRefiner server (https://zhanggroup.org/ModRefiner/).