Log In Sign up
The largest database of trusted experimental protocols

Alexa fluor conjugated phalloidin 546 or 633

Manufactured by Thermo Fisher Scientific
Visit Supplier

Alexa Fluor-conjugated Phalloidin-546 or-633 is a fluorescent probe used for the detection and visualization of F-actin in cells and tissues. It binds specifically to F-actin and can be detected using fluorescence microscopy.

Automatically generated - may contain errors

Lab products found in correlation

Goat or horse serum, by Vector Laboratories (2 mentions) Slowfade, by Thermo Fisher Scientific (2 mentions) Alexa 488, by Thermo Fisher Scientific (2 mentions) Fluoromount, by Thermo Fisher Scientific (2 mentions)

2 protocols using alexa fluor conjugated phalloidin 546 or 633

1

Calbindin 28kDa Immunofluorescence Staining

Check if the same lab product or an alternative is used in the 5 most similar protocols
Following fixation, cultures were permeabilized in 0.1M PBS containing 0.02% Triton X-100 (SIGMA) for 30 minutes at room temperature and subsequently blocked for 1 hour with 10% goat or horse serum (Vector Laboratories). Tissue was incubated in primary antibody overnight at 4°C at the listed dilutions. Antibody staining was visualized using secondary antibodies conjugated to either Alexa488 orAlexa 546 (Invitrogen). Cultures were incubated with all secondary antibodies for 1 hour at room temperature, washed in PBS and then incubated for an additional hour with either Alexa Fluor-conjugated Phalloidin-546 or-633 (Invitrogen) to label filamentous actin. Tissue samples were mounted using either Fluoromount (Invitrogen) or SlowFade (Invitrogen). The following primary antibody was used in this study: Mouse monoclonal antibody againstCalbindin 28kDa protein (1:500, Abcam, ab82812).
Mann Z.F., Thiede B., Chang W., Shin J.B., May-Simera H.L., Lovett M., Corwin J.T, & Kelley M.W. (2014). A gradient of Bmp7 specifies the tonotopic axis in the developing inner ear. Nature communications, 5, 3839.
+ Open protocol
+ Expand
2

Calbindin 28kDa Immunofluorescence Staining

Check if the same lab product or an alternative is used in the 5 most similar protocols
Following fixation, cultures were permeabilized in 0.1M PBS containing 0.02% Triton X-100 (SIGMA) for 30 minutes at room temperature and subsequently blocked for 1 hour with 10% goat or horse serum (Vector Laboratories). Tissue was incubated in primary antibody overnight at 4°C at the listed dilutions. Antibody staining was visualized using secondary antibodies conjugated to either Alexa488 orAlexa 546 (Invitrogen). Cultures were incubated with all secondary antibodies for 1 hour at room temperature, washed in PBS and then incubated for an additional hour with either Alexa Fluor-conjugated Phalloidin-546 or-633 (Invitrogen) to label filamentous actin. Tissue samples were mounted using either Fluoromount (Invitrogen) or SlowFade (Invitrogen). The following primary antibody was used in this study: Mouse monoclonal antibody againstCalbindin 28kDa protein (1:500, Abcam, ab82812).
Mann Z.F., Thiede B., Chang W., Shin J.B., May-Simera H.L., Lovett M., Corwin J.T, & Kelley M.W. (2014). A gradient of Bmp7 specifies the tonotopic axis in the developing inner ear. Nature communications, 5, 3839.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!