The following antibodies were from BD biosciences: FITC Rat anti-mouse CD4 (553651), FITC Rat anti-mouse CD8a (553031), FITC-conjugated hamster anti-mouse CD11c (553801), FITC Rat anti-mouse CD19 (553785), PE anti-mouse CD25 (553075), FITC Rat anti-mouse CD49b (561067), PE Hamster anti-mouse CD69 (553237), APC Rat anti-mouse CD86 (558703), PE Rat anti-mouse CD314 (558403), FITC labeled anti-mouse NK1.1 (553164), Alexa Fluor 647 Rat anti-mouse Foxp3 (560402) and FITC Rat anti-mouse H-2Kb (553569). The following antibodies were from R & D system: Rat anti mouse RAE-1 (MAB17581), Rat anti mouse H60 (MAB1155), Rat anti mouse MULT-1 (MAB2588). FITC goat anti-Rat (A24544) and Alexa Fluor 647 Goat anti-Rat (bs-0293G-AF647) were obtained from Novex and BIOSYNTHESIS, respectively. A CpG ODN (5'-TCGCGAACGTTCGCCGCGTTCGAACGCGG-3′) with full-phosphorothioate modification was synthesized in Takara Biotechnology Company (Dalian, China) and diluted in PBS buffer with no detectable endototin (Limulus amebocyte lysate assay, Associates of Cape Cod, Inc.). CpG ODN-conditioned supernatant was prepared by culturing mouse lymph node cells with CpG ODN at 6μg/ml for 48 hours and collecting the culture medium. All reagents used in this study were pyrogen-free.
Pe hamster anti mouse cd69
Manufactured by BD
The PE Hamster anti-mouse CD69 is a laboratory reagent used for the detection and analysis of CD69 expression on mouse cells. CD69 is a cell surface glycoprotein that is rapidly induced on various cell types upon activation. This reagent can be used in flow cytometry and other immunoassays to identify and characterize CD69-expressing cells.
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Lab products found in correlation
Limulus amebocyte lysate assay, by Associates of Cape Cod (1 mentions)
Alexa fluor 647 rat anti mouse foxp3, by BD (1 mentions)
Facs lysing solution, by BD (1 mentions)
Facsverse, by BD (1 mentions)
Brilliant stain buffer, by BD (1 mentions)
Apc hamster anti mouse cd3e, by BD (1 mentions)
Mouse th17 treg phenotyping kit, by BD (1 mentions)
Cd115 apc, by BioLegend (1 mentions)
Ly6c percp, by BD (1 mentions)
2 protocols using pe hamster anti mouse cd69
1
Immune Cell Phenotyping Protocol
2
Quantification of Circulating Monocytes and Lymphocytes
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Circulating monocytes were determined in 10 ml of heparinised whole blood incubated for 30 min at RT with Ly6C-PerCP (BD Pharmingen, Madrid, Spain) and CD115-APC (Biolegend, San Diego, CA, USA). For lymphocytes, 10 ml of heparinised whole blood was incubated for 30 min RT with 5 ml Brilliant Stain Buffer (563794, BD) and with Brilliant violet (BV)-rat anti-mouse CD4 (562891, BD), BV-rat anti-mouse CD8a (563068, BD), PE-hamster anti-mouse CD69 (553237, BD) and APC-hamster antimouse CD3e (553066, BD). Incubation with lysing solution (BD Facs Lysing solution) was done before analysis by flow cytometry (FACSVerse BD Biosciences). To detect functionally-polirised CD4CT lymphocyte, 100 ml of heparinised whole blood was stained with the mouse Th17/Treg phenotyping kit (BD Pharmingen, Madrid, Spain) to detect CD4CFoxp3C and CD4CIL17 cells. Analysis of Ly6C low -and Ly6C hi -subsets were determined in CD115C populations.
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