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One step rapid mycoplasma detection kit

Manufactured by Yeasen
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The One‐step rapid mycoplasma detection kit is a laboratory equipment product that provides a rapid and efficient method for the detection of mycoplasma contamination in cell cultures. The kit utilizes a straightforward one-step procedure to identify the presence of mycoplasma without the need for extensive sample preparation or specialized equipment.

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Lab products found in correlation

Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions) Penicillin streptomycin mixture, by Wuhan Servicebio Technology (1 mentions) Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (1 mentions) Caski, by American Type Culture Collection (1 mentions)

Close spelling variants of this product

One-Step Mycoplasma Detection Kit Mycoplasma detection kit

2 protocols using one step rapid mycoplasma detection kit

1

Culturing Human Cervical Cancer Cell Lines

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Cervical cancer cell lines CasKi, SiHa, and HeLa were purchased from American Type Culture Collection and authenticated at China Center for Type Culture Collection (Wu Han University, China). Cells were cultured in Dulbecco's Modified Eagle Medium (DMEM, Gibco) containing 10% fetal bovine Serum (Gibco) and 1% penicillin–streptomycin mixture (Servicebio). Cells were kept in an incubator containing 5% CO2 atmosphere at 37°C. All the cells were tested to be free of mycoplasma contamination by a one‐step rapid mycoplasma detection kit (40612ES25, Yeasen).
Wang L., Zhou Y., Cao C., Lin S., Zhi W., Zhang D., Li J., Wei R., Jiang G., Xu H., Wang X., Xi L, & Wu P. (2022). The exon 12‐containing LHX6 isoforms promote cervical cancer cell proliferation by regulating the MAPK signaling pathway. Cancer Medicine, 11(19), 3657-3673.
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2

Rapid Mycoplasma Detection in Cancer Cells

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The cells (DLD1-Control, DLD1-GFP, DLD1-HOXB8, SW480-Control, SW480-shLUC, SW480-shHOXB8-1, SW480-shHOXB8-2, HCT116-Control, HCT116-shLUC, HCT116-shHOXB8-1, HCT116-shHOXB8-2) grew to 80%–90% and cultured for 3 days continually, got the medium to color one-step rapid mycoplasma detection kit (Yeasen) according to the manufacturer’s instructions. If the cancer cells were infected by mycoplasma, the color will turn to navy blue as the positive control, otherwise, it will turn to blue purple as negative control. The result was showed in Additional file 1: Figure S1.
Wang T., Lin F., Sun X., Jiang L., Mao R., Zhou S., Shang W., Bi R., Lu F, & Li S. (2019). HOXB8 enhances the proliferation and metastasis of colorectal cancer cells by promoting EMT via STAT3 activation. Cancer Cell International, 19, 3.
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