The seed pretreatment and environmental (light and humidity) conditions for germination were consistent with those used for the germination test. The difference in germination rate at different germination stages at 30/20°C was used as the sampling standard. Samples were collected at 3 d (S1-germination rate, 0), 7 d (S2-germination rate, 10%), 9 d (S3-germination rate, 30%), 10 d (S4-germination rate, 50%), and 13 d (S5-germination rate, 80%) after seeding (Figure 1 ). The seeds from three germinating boxes were randomly selected each time as three replicates, quickly frozen using liquid nitrogen, and stored at −80°C for subsequent experiments. Total RNA was extracted using a plant RNA kit (OMEGA, GA, United States). The quantity and integrity of the RNA samples were assessed using a Drop ND-1000 spectrophotometer (NanoDrop Technologies, Wilmington, DE, United States) and 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA, United States), respectively.
Drop nd 1000 spectrophotometer
Manufactured by Thermo Fisher Scientific
Sourced in United States
The Drop ND-1000 spectrophotometer is a laboratory instrument used to measure the absorbance or transmittance of samples across a range of wavelengths. It is designed to provide accurate and reliable measurements for various applications in scientific research and analysis.
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Lab products found in correlation
2 protocols using drop nd 1000 spectrophotometer
1
Germination Transcriptome Profiling
2
Transcriptome Analysis of Plant Leaves
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In 21 July 2020, we collected green, lilac and purple three kinds of leaves of different branches on a single plant as experimental materials in the breeding base of Beijing Academy of Agriculture and Forestry Sciences, China. (Figure. 1) . We also collected roots, flowers, stalks as materials of total RNA libraries. After quick-freezing in the liquid nitrogen, the collected leaves were stored at -80 °C for subsequent experiments.
Using a plant RNA kit (OMEGA, Georgia, USA, No. R6827-01) to extract total RNA. RNA samples quantity and integrity were tested in Drop ND-1000 spectrophotometer (NanoDrop Technologies, Delaware, USA) and 2100 Bioanalyzer (Agilent Technologies, California, USA) (Zhang et al., 2016) , respectively.
Using a plant RNA kit (OMEGA, Georgia, USA, No. R6827-01) to extract total RNA. RNA samples quantity and integrity were tested in Drop ND-1000 spectrophotometer (NanoDrop Technologies, Delaware, USA) and 2100 Bioanalyzer (Agilent Technologies, California, USA) (Zhang et al., 2016) , respectively.
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