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22 gauge needle

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The 22-gauge needle is a medical device used for various procedures that require the injection or withdrawal of fluids or substances. It has a diameter of 0.7 millimeters and is commonly used in medical settings such as hospitals, clinics, and laboratories.

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Lab products found in correlation

Collagenase d, by Roche (7 mentions) Lymphocyte separation medium, by MP Biomedicals (7 mentions) Easysep human cd34 positive selection kit, by STEMCELL (5 mentions) Anti human cd34 microbeads, by Miltenyi Biotec (4 mentions) X rad 320, by Hamilton Company (2 mentions) X rad 320 irradiator, by Hamilton Company (2 mentions) Macs cd34 micro bead kit, by Miltenyi Biotec (1 mentions) Amg487, by Bio-Techne (1 mentions) Pifithrin α pft α, by Merck Group (1 mentions)

11 protocols using 22 gauge needle

1

Human Fetal Liver Hematopoietic Cell Transplantation in Mice

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De-identified human fetal liver tissues, obtained with informed consent from the donors, were procured by Advanced Bioscience Resources, Inc. and their use was determined as non-human subject research by Fred Hutch’s Institutional Review Board (6007–827). Fetal livers were cut in small fragments, treated for 45 min at 37°C with collagenase D (Roche, 100 ng/ml), and a cell suspension was prepared. Hematopoietic cells were enriched by density gradient centrifugation (Lymphocyte Separation Medium, MP Biomedicals) followed by positive immunomagnetic selection with anti-human CD34 microbeads (Miltenyi Biotec). Purity (>90% CD34+ cells) was confirmed by flow cytometry and cells were frozen at −80°C in FBS containing 10% DMSO.
Newborn mice (day 1–3) were sublethally irradiated (80 cGy gamma rays in a Cesium-137 irradiator) and ~20,000 CD34+ cells in 20 μl of PBS were injected into the liver with a 22-gauge needle (Hamilton Company), as previously described [25 (link), 26 (link)]. Engraftment levels were measured as the percentage of human CD45+ cells among total (mouse and human combined) CD45+ cells in the blood. Mice with engraftment levels lower than 10% were excluded from further experimentation.
Voillet V., Berger T.R., McKenna K.M., Paulson K.G., Tan W.H., Smythe K.S., Hunter D.S., Valente W.J., Weaver S., Campbell J.S., Kim T.S., Byrd D.R., Bielas J.H., Pierce R.H., Chapuis A.G., Gottardo R, & Rongvaux A. (2022). An in vivo model of human macrophages in metastatic melanoma. Journal of immunology (Baltimore, Md. : 1950), 209(3), 606-620.
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2

Fetal Liver CD34+ Cell Engraftment

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Fetal liver samples were cut in small fragments, treated for 45 min at 37°C with collagenase D (Roche, 200 μg/ml), and prepared into a cell suspension. Human CD34+ cells were purified by performing density gradient centrifugation (Lymphocyte Separation Medium, MP Biomedicals), followed by positive immunomagnetic selection with EasySep™ Human CD34 Positive Selection Kit (Stemcell). For intra-hepatic engraftment, newborn 1–3 day-old pups were injected with 20,000 fetal liver CD34+ cells in 20 μl of PBS were injected into the liver with a 22-gauge needle (Hamilton Company). All use of human materials was approved by the Yale University Human Investigation Committee.
Sefik E., Israelow B., Mirza H., Zhao J., Qu R., Kaffe E., Song E., Halene S., Meffre E., Kluger Y., Nussenzweig M., Wilen C.B., Iwasaki A, & Flavell R.A. (2021). A humanized mouse model of chronic COVID-19. Nature biotechnology, 40(6), 906-920.
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3

Fetal Liver CD34+ Cell Engraftment

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Fetal liver samples were cut in small fragments, treated for 45 min at 37 °C with collagenase D (Roche, 200 μg/ml), and prepared into a cell suspension. Human CD34+ cells were purified by performing density gradient centrifugation (Lymphocyte Separation Medium, MP Biomedicals), followed by positive immunomagnetic selection with EasySep™ Human CD34 Positive Selection Kit (StemCell). For intrahepatic engraftment, newborn 1–3-day-old pups were injected with 20,000 fetal liver CD34+ cells in 20 μl of PBS into the liver with a 22-gauge needle (Hamilton Company). All use of human materials was approved by the Yale University Human Investigation Committee.
Sefik E., Qu R., Junqueira C., Kaffe E., Mirza H., Zhao J., Brewer J.R., Han A., Steach H.R., Israelow B., Blackburn H.N., Velazquez S.E., Chen Y.G., Halene S., Iwasaki A., Meffre E., Nussenzweig M., Lieberman J., Wilen C.B., Kluger Y, & Flavell R.A. (2022). Inflammasome activation in infected macrophages drives COVID-19 pathology. Nature, 606(7914), 585-593.
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4

Fetal Liver CD34+ Cell Transplantation

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Fetal liver samples were cut in small fragments, treated for 45 min at 37 °C with collagenase D (Roche, 200 μg/ml), and prepared into a cell suspension. Human CD34+ cells were purified by performing density gradient centrifugation (Lymphocyte Separation Medium, MP Biomedicals), followed by positive immunomagnetic selection with EasySep ™ Human CD34 Positive Selection Kit (Stemcell). For intra-hepatic engraftment, newborn 1–3 day-old pups were injected with 20,000 fetal liver CD34+ cells in 20 μl of PBS were injected into the liver with a 22-gauge needle (Hamilton Company). All use of human materials was approved by the Yale University Human Investigation Committee.
Sefik E., Israelow B., Zhao J., Qu R., Song E., Mirza H., Kaffe E., Halene S., Meffre E., Kluger Y., Nussenzweig M., Wilen C.B., Iwasaki A, & Flavell R.A. (2021). A humanized mouse model of chronic COVID-19 to evaluate disease mechanisms and treatment options. Research Square.
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5

Xenotransplantation of human hematopoietic stem cells

Cited 1 time
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Primary hHSPCs (CD34+) cells were purified from the BM or peripheral blood of patients using Ficoll density gradient centrifugation and further magnetically isolated using the MACS CD34 MicroBead Kit (Miltenyi Biotec). CD34+ cells were cryopreserved and slowly thawed in IMDM with 50% FCS at 37 °C before xenotransplantation and resuspended in 25 µl PBS for injection. MISTRG mice aged 8–12 weeks old59 (link) were irradiated sub-lethally (181 cGy using an X-ray RS-2000 irradiator, Rad Source) and transplanted with a 22-gauge needle (Hamilton Company) intrafemorally with 1.5–3 × 105 CD34+ hHSPCs.
Grockowiak E., Korn C., Rak J., Lysenko V., Hallou A., Panvini F.M., Williams M., Fielding C., Fang Z., Khatib-Massalha E., García-García A., Li J., Khorshed R.A., González-Antón S., Baxter E.J., Kusumbe A., Wilkins B.S., Green A., Simons B.D., Harrison C.N., Green A.R., Lo Celso C., Theocharides A.P, & Méndez-Ferrer S. (2023). Different niches for stem cells carrying the same oncogenic driver affect pathogenesis and therapy response in myeloproliferative neoplasms. Nature Cancer, 4(8), 1193-1209.
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6

Fetal Liver CD34+ Cell Engraftment

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Fetal liver samples were cut in small fragments, treated for 45 min at 37°C with collagenase D (Roche, 200 μg/ml), and prepared into a cell suspension. Human CD34+ cells were purified by performing density gradient centrifugation (Lymphocyte Separation Medium, MP Biomedicals), followed by positive immunomagnetic selection with EasySep™ Human CD34 Positive Selection Kit (Stemcell). For intra-hepatic engraftment, newborn 1–3 day-old pups were injected with 20,000 fetal liver CD34+ cells in 20 μl of PBS were injected into the liver with a 22-gauge needle (Hamilton Company). All use of human materials was approved by the Yale University Human Investigation Committee.
Sefik E., Israelow B., Mirza H., Zhao J., Qu R., Kaffe E., Song E., Halene S., Meffre E., Kluger Y., Nussenzweig M., Wilen C.B., Iwasaki A, & Flavell R.A. (2021). A humanized mouse model of chronic COVID-19. Nature biotechnology, 40(6), 906-920.
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7

Humanized Mouse Model via Fetal Liver CD34+ Cells

Cited 2 times
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Recipient mice were engrafted with human hematopoietic progenitor cells as previously described (14 (link), 71 (link)). Briefly, human fetal liver samples were cut into small fragments and treated with collagenase D (Roche) 100 ng/mL for 45 min at 37 °C. Human CD34+ cells from the resulting cell suspension were purified from the fetal liver by density gradient centrifugation (Lymphocyte Separation Medium, MP Biomedicals) followed by positive immunomagnetic selection with the EasySepTM Human CD34 Positive Selection Kit (StemCell). Cells were frozen in FBS (fetal bovine serum) containing 10% dimethyl sulfoxide (DMSO) and stored in liquid nitrogen. For intrahepatic engraftment, newborn 1 to 3-d-old pups were irradiated with 80 rad using a cabinet irradiator (X-RAD 320) and then injected with 10,000 fetal liver CD34+ cells in PBS into the liver with a 22-gauge needle (Hamilton Company).
Odell I.D., Agrawal K., Sefik E., Odell A.V., Caves E., Kirkiles-Smith N.C., Horsley V., Hinchcliff M., Pober J.S., Kluger Y, & Flavell R.A. (2023). IL-6 trans-signaling in a humanized mouse model of scleroderma. Proceedings of the National Academy of Sciences of the United States of America, 120(37), e2306965120.
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8

Fetal Liver CD34+ Cell Engraftment

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Fetal liver samples were cut in small fragments, treated for 45 min at 37 °C with collagenase D (Roche, 200 μg/ml), and prepared into a cell suspension. Human CD34+ cells were purified by performing density gradient centrifugation (Lymphocyte Separation Medium, MP Biomedicals), followed by positive immunomagnetic selection with EasySep™ Human CD34 Positive Selection Kit (StemCell). For intrahepatic engraftment, newborn 1–3-day-old pups were injected with 20,000 fetal liver CD34+ cells in 20 μl of PBS into the liver with a 22-gauge needle (Hamilton Company). All use of human materials was approved by the Yale University Human Investigation Committee.
Sefik E., Qu R., Junqueira C., Kaffe E., Mirza H., Zhao J., Brewer J.R., Han A., Steach H.R., Israelow B., Blackburn H.N., Velazquez S.E., Chen Y.G., Halene S., Iwasaki A., Meffre E., Nussenzweig M., Lieberman J., Wilen C.B., Kluger Y, & Flavell R.A. (2022). Inflammasome activation in infected macrophages drives COVID-19 pathology. Nature, 606(7914), 585-593.
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9

Engrafting Human CD34+ Cells in NSG Mice

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Human CD34+ cells were purified from fetal liver samples by density gradient centrifugation followed by positive immunomagnetic selection with anti-human CD34 microbeads (Miltenyi Biotech). Newborn NSG mice (within first 3 days of life) were sublethally irradiated (x-ray irradiation with X-RAD 320 irradiator at 180 cGy), and 100,000 to 150,000 CD34+ cells in 20 µl of phosphate-buffered saline were injected into the liver with a 22-gauge needle (Hamilton Company).Mice were used for experiments 10 to 12 weeks after transplantation. NSG mice treated with the PTPN22 inhibitor were injected with the PTPN22 inhibitor 0.75 or 0.15 mg ip twice daily for a week. All animals were treated, and experiments were conducted in accordance with the Yale institutional reviewed guidelines on treatment of experimental animals.
Schickel J.N., Kuhny M., Baldo A., Bannock J.M., Massad C., Wang H., Katz N., Oe T., Menard L., Soulas-Sprauel P., Strowig T., Flavell R, & Meffre E. (2016). PTPN22 inhibition resets defective human central B cell tolerance. Science immunology, 1(1), aaf7153.
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10

Humanized NSG Mouse Model

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Human CD34+ cells were purified from fetal liver samples by density-gradient centrifugation followed by positive immunomagnetic selection with anti-human CD34 microbeads (Miltenyi Biotech). Newborn NSG mice (within the first 3 d of life) were sublethally irradiated (x-ray irradiation with X-RAD 320 irradiator at 180 cGy) and 100,000–150,000 CD34+ cells in 20 µl of PBS were injected into the liver with a 22-gauge needle (Hamilton Company). Mice were used for experiments 10–12 wk after transplantation. NSG mice treated with the HCQ were injected with the HCQ 0.2 mg/dose intraperitoneally daily for a week. NSG mice treated with the CXCR3 antagonist were injected with the AMG-487 5 mg/kg/dose (Tocris) intraperitoneally daily for 9 d.
All animals were treated and experiments were conducted in accordance with the Yale institutional review guidelines on the treatment of experimental animals.
Çakan E., Ah Kioon M.D., Garcia-Carmona Y., Glauzy S., Oliver D., Yamakawa N., Vega Loza A., Du Y., Schickel J.N., Boeckers J.M., Yang C., Baldo A., Ivashkiv L.B., Young R.M., Staudt L.M., Moody K.L., Nündel K., Marshak-Rothstein A., van der Made C.I., Hoischen A., Hayward A., Rossato M., Radstake T.R., Cunningham-Rundles C., Ryu C., Herzog E.L., Barrat F.J, & Meffre E. (2023). TLR9 ligand sequestration by chemokine CXCL4 negatively affects central B cell tolerance. The Journal of Experimental Medicine, 220(12), e20230944.
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