5 5 dithiobis 2 nitrobenzoic acid dtnb

Manufactured by Sisco Research Laboratories

Sourced in India

5,5′-dithiobis-(2-nitrobenzoic acid) (DTNB) is a chemical compound used as a reagent in various biochemical assays. It is a colorimetric agent that reacts with thiol groups, producing a yellow-colored product that can be detected spectrophotometrically. The absorbance of the colored product is proportional to the concentration of thiol groups present in the sample, making DTNB a useful tool for the quantification of sulfhydryl-containing compounds.

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5,5-dithiobis(2-nitrobenzoic acid), (14 citations)

7 protocols using 5 5 dithiobis 2 nitrobenzoic acid dtnb

Dextran-Chitosan Nanocarrier for Anticancer Drug Delivery

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Dextran (MW ~70,000), chitosan (medium molecular weight), l-cysteine,
N-(3-dimethylaminopropyl)-N′-ethylcarbodiimide
hydrochloride (EDAC), dialysis tubing (molecular weight cut-off (MWCO) 12,000
Da), phalloidin–fluorescein isothiocyanate (FITC), 4′,6-diamidino-2-phenylindole
(DAPI) stain, PVA-DABCO^® antifadding agent, 2′,7′-dichlorofluorescein
diacetate (DCFDA), and doxorubicin hydrochloride were purchased from
Sigma-Aldrich, USA. Anti-collagen mouse mAb and cy5-tagged anti-mouse secondary
antibody were purchased from Cell Signalling Technology, Inc. Sodium periodate,
diethylene glycol (DEG), and 5,5′-dithio-bis-(2-nitrobenzoic acid) (DTNB) were
procured from SRL chemicals, India. Lung adenocarcinoma A549 cells were obtained
with passage no 21 from NCCS, India. Fetal bovine serum (FBS) was procured from
Gibco, USA. Bovine serum albumin (BSA), Roswell Park Memorial Institute
(RPMI)-1640, and penicillin–streptomycin antibiotic solution were purchased from
Himedia, India. LIVE/DEAD^® Viability/Cytotoxicity Kit was purchased
from Thermo Fisher Scientific, India.

Kedaria D, & Vasita R. (2017). Bi-functional oxidized dextran–based hydrogel inducing microtumors: An in vitro three-dimensional lung tumor model for drug toxicity assays. Journal of Tissue Engineering, 8, 2041731417718391.

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Locust Bean Gum-Chitosan Hydrogel Synthesis

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Locust bean gum (from Ceratonia siliqua seeds, Molecular Weight ∼ 310 kDa, M/G = 4:1), chitosan (Molecular weight 190–310 kDa and Degree of deacetylation 75–85%), dextran (from Leuconostoc ssp., Mr (Relative molecular mass) ∼ 70 kDa), L-cysteine, N-(3-Dimethylaminopropyl)-N′-ethylcarbodiimide hydrochloride (EDAC), dialysis tubing cellulose membrane (MWCO ∼ 12,000 Da), were purchased from Sigma –Aldrich. 5, 5′-dithio-bis-(2-nitrobenzoic acid) (DTNB), diethylene glycol (DEG) and sodium periodate were purchased from SRL chemicals, India. Mouse embryonic fibroblast 3T3-L1 cells were received from NCCS Pune, India. DMEM and Fetal bovine serum (FBS) were purchased from Gibco, USA.

Meena L.K., Raval P., Kedaria D, & Vasita R. (2017). Study of locust bean gum reinforced cyst-chitosan and oxidized dextran based semi-IPN cryogel dressing for hemostatic application. Bioactive Materials, 3(3), 370-384.

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Biochemical Assays for Neurological Disorders

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Acetylthiocholine iodide, adenine, ketamine, xylazine, thiobarbituric acid, 1,1,3,3-tetramethoxypropane, 3,3-diaminobenzidine (DAB) liquid substrate system, hydrogen peroxide (H₂O₂), Sirius red and poly-L-lysine were obtained from Sigma-Aldrich Co., USA. Potassium dichromate, silver nitrate and formaldehyde were obtained from HiMedia laboratories Pvt. Ltd., India. Acetic acid, cresyl violet, Nitro blue tetrazolium (NBT), 5,5′-dithiobis-(2-nitrobenzoic acid) (DTNB), cytochrome c, pyrogallol, salicylic acid, tri-sodium citrate, copper sulphate, potassium ferricyanide, paraformaldehyde, picric acid, Triton X-100, sodium dodecyl sulphate and other chemicals were of analytical reagent grade, procured from SISCO Research Laboratories Pvt. Ltd., India. Rabbit anti-Glial fibrillary acidic protein (GFAP) antibody (ab7260) and rabbit anti-tyrosine hydroxylase (TH) antibody (ab112) were procured from Abcam (Cambridge, UK). Anti-rabbit goat secondary antibody conjugated with horseradish peroxidase (HRP; ap307p) was procured from Millipore Co. (USA). Urea, creatinine and uric acid assay kits were obtained from Coral Clinical Systems, Goa, India (Reference no. 1102240075, 1101070275, 1102260275).

Mazumder M.K., Paul R., Bhattacharya P, & Borah A. (2019). Neurological sequel of chronic kidney disease: From diminished Acetylcholinesterase activity to mitochondrial dysfunctions, oxidative stress and inflammation in mice brain. Scientific Reports, 9, 3097.

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Cellular Antioxidant Activity Assay

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5,5′-Dithiobis-(2-nitrobenzoic acid)-DTNB, ethylene diamine tetra acetic acid (EDTA), horse radish peroxidase (HRP), glutathione reduced, pyrogallol and heparin were obtained from Sisco Research Laboratories (SRL), India. Histopaque-1077, RPMI-1640 medium, low melting agarose and thiobarbituric acid (TBA) were purchased from Sigma Chemicals Co, USA. Trichloro acetic acid (TCA) was obtained from Qualigens Fine Chemicals, India while ethidium bromide (EtBr) from Hi Media, India Ltd. All other chemicals were of highest analytical grade available.

Khan M., Naqvi A.H, & Ahmad M. (2015). Comparative study of the cytotoxic and genotoxic potentials of zinc oxide and titanium dioxide nanoparticles. Toxicology Reports, 2, 765-774.

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Antioxidant Assays and Reagents

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2,2′-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) was purchased from Roche diagnostics, Germany and 6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid (Trolox) was obtained from Fluka, Switzerland. Bathophenanthrolinesulfonate disodium salt, 5,5′-dithiobis-2-nitrobenzoic acid (DTNB) and N-(1-Naphthyl) ethylenediaminedihydrochloride (NED) were procured from Sisco Research Laboratories Pvt. Ltd, India. 1-chloro-2,4-dinitrobenzene (CDNB), Dimethyl-4-aminobenzaldehyde, and N,N- dimethyl-4-nitrosoaniline ammonium iron (II) sulfate hexahydrate ((NH₄)₂Fe(SO₄)_2, 6H₂O) were obtained from Merck, Mumbai, India. Guanidine hydrochloride and Iron-dextran was purchased from Sigma-Aldrich, USA. Desirox (Deferasirox) was obtained from Cipla Ltd., India. All of the other used reagents were of molecular biology grade and were obtained from reputable suppliers.

Chaudhuri D., Ghate N.B., Panja S., Basu T., Shendge A.K, & Mandal N. (2016). Glycoside rich fraction from Spondias pinnata bark ameliorate iron overload induced oxidative stress and hepatic damage in Swiss albino mice. BMC Complementary and Alternative Medicine, 16, 262.

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Purification and Analysis of α-Crystallin

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Dithiothreitol (DTT); 4,4′-dianilino-1,1′-binaphthyl-5,5′-disulfonic
acid, dipotassium salt (bis-ANS); guanidium hydrochloride (GdnHCl);
and ethylenediaminetetraacetic acid, disodium salt dehydrate (EDTA)
were obtained from Sigma Chemical Co. (St. Louis, MO). 5,5′-Dithiobis(2-nitrobenzoic
acid) (DTNB) was obtained from Sisco Research Laboratories, India.
α_L-Crystallin was purified from bovine eye lenses,
as previously described.^{21 (link)} All other chemicals
were of analytical grade.

DiMauro M.A., Nandi S.K., Raghavan C.T., Kar R.K., Wang B., Bhunia A., Nagaraj R.H, & Biswas A. (2014). Acetylation of Gly1 and Lys2 Promotes Aggregation of Human γD-Crystallin. Biochemistry, 53(46), 7269-7282.

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Antioxidant and Cardioprotective Compounds

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Nitro blue tetrazolium (NBT), glutathione (GSH) and 5-5′ dithiobis (2-nitro benzoic acid) (DTNB) were purchased from Sisco Research Laboratories Pvt. Ltd, Mumbai, India; thiobarbituric acid from Hi Media Laboratories, Mumbai, India, doxorubicin from Naprod Life Sciences Pvt Ltd. and chrysin, apigenin, luteolin, quercetin, gallic acid and kaempferol (analytical grade standard compounds) from Sigma Aldrich Corporation (St. Luis, MO, USA). Biochemical kits manufactured by Euro Diagnostic Systems, Chennai, India, were used for estimation of Lactate Dehydrogenase (LDH), Creatine Phosphokinase (CPK), Serum Glutamate Oxaloacetate Transaminase (SGOT) and Serum Glutamate Pyruvate Transaminase (SGPT). All other chemicals and reagents used were of analytical grade.

Menon S., Lawrence L., Sivaram V.P, & Padikkala J. (2018). Oroxylum indicum root bark extract prevents doxorubicin-induced cardiac damage by restoring redox balance. Journal of Ayurveda and Integrative Medicine, 10(3), 159-165.

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