All of the chemicals and solvents were purchased from Sigma-Aldrich (St. Louis, MO, USA) and TCI America company (Portland, OR, USA) unless indicated otherwise. Poly(9,9-dioctylfluorene) (PFO) (average MW 147 000, polydispersity 3.0) was purchased from ADS Dyes, Inc. (Quebec, Canada). BODIPY 520 dye, BODIPY 680 dye, and BODIPY 520-fluorene copolymer were synthesized according to our previously published protocols.1 (link) Polystyrene grafted ethylene oxide functionalized with carboxyl groups (PS-PEGCOOH, main chain MW 8500, graft chain MW 1200, total chain MW 21 700, polydispersity 1.25) was purchased from Polymer Source Inc. (Quebec, Canada). Streptavidin was purchased from Invitrogen (Eugene, OR, USA). Quantum Dot 525 was purchased from Life Technologies Corporation of Thermo Fisher Scientific (Carlsbad, CA, USA). All chemicals were used as received without further purification. High purity Milli-Q water (18.2 MΩ·cm) was used throughout the experiments.
Ps peg cooh
Manufactured by Polymer Source
Sourced in Canada
PS-PEG-COOH is a polymer composed of polystyrene (PS) and polyethylene glycol (PEG) with a carboxyl (COOH) functional group. It is a synthetic material commonly used in laboratory applications.
Automatically generated - may contain errors
Lab products found in correlation
Hydrochloric acid (hcl), by Guangzhou Chemical (3 mentions)
Sodium hydroxide (naoh), by Guangzhou Chemical (3 mentions)
Tetrahydrofuran hplc grade, by Merck Group (1 mentions)
Absolute ethanol, by Avantor (1 mentions)
Amicon ultra 15, by Merck Group (1 mentions)
Tetrahydrofuran thf, by Avantor (1 mentions)
Hepes, by Thermo Fisher Scientific (1 mentions)
Alexa 647, by BD (1 mentions)
Alexa 488, by BD (1 mentions)
Fluorescein isothiocyanate (fitc), by BD (1 mentions)
6 protocols using ps peg cooh
1
Fluorescent Polymer Synthesis and Functionalization
2
Synthesis and Characterization of Polymer Nanocomposites
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PFV (95:5 mole ratio), BDMO-PPV, HPLC grade tetrahydrofuran (THF) and (3-Aminopropyl)- triethoxysilane (APTES) were purchased from Sigma-Aldrich (Milwankee, USA). PS-PEG-COOH was purchased from Polymer Source Inc. Ltd., (Dorval, QC, Canada; Back bone PS Mn = 6500; each branch Mn = 4600; Mn total = 30 000, Mw/Mn = 1.3). Hydrochloric acid (AR, 36.5%) and sodium hydroxide (AR) were ordered from Guangzhou Chemical Reagent Factory (Guangzhou, China). Ultra-pure water used for all the experiments was prepared with a Milli-Q water system (Millipore, Bedford, MA, USA).
3
Hydrogenase-Polymer Hybrid Nanostructures
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Polymer poly(9,9-dioctylfluorene-alt-bithiophene), also known as F8T2 (coded as P1 in this work, Mw 64 kDa), was purchased
from Ossila, U.K. Triblock copolymer (P2 , Mw 20–30 kDa) poly(N,N-dimethylamino ethyl methacrylate)-B-poly(9,9-N-dihyxyl-2,7-fluorene)-B-poly(N,N-dimethylamino ethyl methacrylate) and
copolymer polystyrene grafted with carboxy-terminated polyethylene
oxide (PS-PEG-COOH, backbone chain Mw 8.5
kDa, graft chain Mw 4.6 kDa, total chain
36.5 kDa) were purchased from Polymer Source Inc., Canada. The hydrogenase
CrHydA1 (Mw 50 kDa) was prepared following
literature protocols, and the specific activity was 325 μmolH2·mgH2ase–1·min–1.44 (link),45 (link) InSupporting Information (SI) details about hydrogenase expression, purification,
reconstitution, and maturation are presented. Centrifugal filters
with molecular weight (Mw) cutoffs of
10 kDa (Amicon Ultra-15) and 100 kDa (Vivaspin 2) were purchased from
Merck and Sigma-Aldrich, respectively. Tetrahydrofuran (THF) and absolute
ethanol (99.5%+) were purchased from VWR Chemicals. Other chemicals
were obtained from Sigma-Aldrich and all chemicals were used without
further purification unless stated otherwise.
from Ossila, U.K. Triblock copolymer (
copolymer polystyrene grafted with carboxy-terminated polyethylene
oxide (PS-PEG-COOH, backbone chain Mw 8.5
kDa, graft chain Mw 4.6 kDa, total chain
36.5 kDa) were purchased from Polymer Source Inc., Canada. The hydrogenase
CrHydA1 (Mw 50 kDa) was prepared following
literature protocols, and the specific activity was 325 μmolH2·mgH2ase–1·min–1.44 (link),45 (link) In
reconstitution, and maturation are presented. Centrifugal filters
with molecular weight (Mw) cutoffs of
10 kDa (Amicon Ultra-15) and 100 kDa (Vivaspin 2) were purchased from
Merck and Sigma-Aldrich, respectively. Tetrahydrofuran (THF) and absolute
ethanol (99.5%+) were purchased from VWR Chemicals. Other chemicals
were obtained from Sigma-Aldrich and all chemicals were used without
further purification unless stated otherwise.
4
Photophysical Characterization of Polymers
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All of the chemicals and solvents were purchased from Sigma-Aldrich (St Louis, MO, USA) unless indicated otherwise. The fluorescent semiconducting polymer CN-PPV (molecular weight (MW) 15,000, polydispersity 5.9) was purchased from ADS Dyes, Inc. (Quebec, Canada). The photochromic quencher BTE was purchased from TCI (Portland, OR, USA). A comb-like polymer—polystyrene grafted with ethylene oxide functionalized with carboxyl groups (PS-PEG-COOH, main chain MW 8,500, graft chain MW 1,200, total chain MW 21,700, polydispersity 1.25)—was purchased from Polymer Source Inc. (Quebec, Canada). The fluorochromes, including FITC, APC, Alexa 488 and Alexa 647, used in the photobleaching experiment were purchased from BD Bioscience (San Jose, CA, USA). HEPES, EDC (1-ethyl-3-[3-dimethylaminopropyl]carbodiimide hydrochloride) and streptavidin were purchased from Invitrogen (Eugene, OR, USA). All chemicals were used as received without further purification. High purity of MilliQ water (18.2 MΩ cm−1) was used throughout the experiment.
5
Synthesis of BBTD Polymer Precursor
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All chemicals were purchased from commercial sources and used without further purification. All solvents were purified before use. PS–PEG–COOH was purchased from Polymer Source Inc. Ttriphenylamine‐benzo[1,2‐c:4,5‐c’]bis([1,2,5]thiadiazole) (BBTD) was synthesized according to the previous report.
6
Semiconducting Polymer and Non-Fullerene Acceptor Synthesis
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Semiconducting polymer poly[(9,9-dioctylfluorenyl-2,7-diyl)-co-(1,4-benzo-{2,1′,3}-thiadiazole)] (F8BT, also
named as PFBT, D1, Mw 376 200)
and n-type nonfullerene acceptor 3,9-bis(2-methylene-(3-(1,1-dicyanomethylene)indanone))-5,5,11,11-tetrakis(4-hexylphenyl)dithieno[2,3-d:2′,3′-d′]-s-indaceno[1,2-b:5,6-b′]dithiophene (ITIC)
were purchased from Ossila, UK. PFODTBT polymer (D2, Mw 50–80 kDa) was purchased from Solaris
Chem. The copolymer, polystyrene grafted with ethylene oxide and carboxyl
groups (PS–PEG–COOH, backbone chain Mw 8500, graft chain Mw 4600,
total chain Mw 36 500), was purchased
from Polymer Source Inc., Canada. All other chemical reagents were
purchased from Sigma-Aldrich and used as received unless indicated
otherwise. All experiments and measurements were carried out at room
temperature unless indicated otherwise.
named as PFBT, D1, Mw 376 200)
and n-type nonfullerene acceptor 3,9-bis(2-methylene-(3-(1,1-dicyanomethylene)indanone))-5,5,11,11-tetrakis(4-hexylphenyl)dithieno[2,3-d:2′,3′-d′]-s-indaceno[1,2-b:5,6-b′]dithiophene (ITIC)
were purchased from Ossila, UK. PFODTBT polymer (D2, Mw 50–80 kDa) was purchased from Solaris
Chem. The copolymer, polystyrene grafted with ethylene oxide and carboxyl
groups (PS–PEG–COOH, backbone chain Mw 8500, graft chain Mw 4600,
total chain Mw 36 500), was purchased
from Polymer Source Inc., Canada. All other chemical reagents were
purchased from Sigma-Aldrich and used as received unless indicated
otherwise. All experiments and measurements were carried out at room
temperature unless indicated otherwise.
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